葡萄籽原花青素对牙龈卟啉单胞菌内毒素的影响

目的探讨葡萄籽原花青素对牙龈卟啉单胞菌内毒素的影响。方法采用二倍稀释法测定葡萄籽原花青素对牙龈卟啉单胞菌的最小抑菌浓度(MIC);采用鲎试验测定低于MIC 6个浓度的葡萄籽原花青素对牙龈卟啉单胞菌内毒素含量的影响。采用SPSS17.0软件包进行组间比较。结果葡萄籽原花青素对牙龈卟啉单胞菌的MIC为0.8 mg/mL;在0.05~0.4 mg/mL范围内,随着葡萄籽原花青素浓度的升高,对释放内毒素的抑制作用逐渐增强（P<0.05、0.01）。结论葡萄籽原花青素对牙龈卟啉单胞菌内毒素的释放具有抑制作用。

Grape seed proanthocyanidin extracts inhibit lipopolysaccharide of Porphyromonas gingivalis

PURPOSE: To study the inhibitory effect of grape seed proanthocyanidin extracts on 1ipopolysacharides of Porphyromonas gingivalis. METHODS: Grape seed proanthocyanidin extracts were to different concentrations by dilution. The effect on Porphyromonas gingivalis was measured by minimal inhibitory concentration (MIC). The inhibition effect of grape seed proanthocyanidin extracts on lipopolysaccharide was measured by limulus assay with 6 concentrations below the minimal inhibitory concentration. SPSS 17.0 software package was used for statistical analysis. RESULTS: The value of MIC of grape seed proanthocyanidin extracts was 0.8 mg/mL to the strains of the experimental bacteria. The inhibitory effect on lipopolysaccharide enhanced with the increasing of the concentration of grape seed proanthocyanidin extracts within the scope of 0.05 to 0.4 mg/mL (P<0.05,0.01). CONCLUSIONS: Grape seed proanthocyanidin extracts have inhibitory effect on lipopolysaccharide of Porphyromonas gingivalis.