| 苹果S6PDH基因克隆与原核表达 |
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| 引用本文： | 梁东,马锋旺,张敏. 苹果S6PDH基因克隆与原核表达[J]. 西北植物学报, 2008, 28(1): 33-36 |
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| 作者姓名： | 梁东 马锋旺 张敏 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西杨陵,712100 |
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| 基金项目： | 陕西省重大科技专项计划项目(2006kz05-G4)，西北农林科技大学“拔尖人才支持计划” |
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| 摘 要： | 6-磷酸山梨醇脱氢酶(sorbitol-6-phosphate dehydrogenase,S6PDH)是蔷薇科植物中合成山梨醇的重要酶。以苹果叶片为材料,利用RT-PCR法克隆到S6PDHcDNA全长,将其与大肠杆菌表达载体pET-32a( )构建原核表达载体pET-S并转化大肠杆菌BL21,经IPTG诱导表达后,SDS-PAGE检测结果表明该基因表达了1个约54kD的蛋白,为进一步研究目的蛋白的结构和功能提供了试验基础。
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| 关 键 词： | 苹果 山梨醇 6-磷酸山梨醇脱氢酶 原核表达 |
| 文章编号： | 1000-4025（2008）01-0033-04 |
| 收稿时间： | 2007-07-27 |
| 修稿时间： | 2007-10-22 |
Cloning and Prokaryotic Expression of Apple S6PDH Gene |
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| LIANG Dong,MA Feng-wang,ZHANG Min. Cloning and Prokaryotic Expression of Apple S6PDH Gene[J]. Acta Botanica Boreali-Occidentalia Sinica, 2008, 28(1): 33-36 |
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| Authors: | LIANG Dong MA Feng-wang ZHANG Min |
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| Abstract: | Sorbitol-6-phosphate dehydrogenase(S6PDH) is a key enzyme for the biosynthesis of sorbitol in Rosaceae plants.S6PDH cDNA was amplified by RT-PCR from apple leaf,then was cloned into pET-32a( ) vector to construct recombinant prokaryotic expression vector pET-S.The pET-S was transformed to E.coli BL21.After induced by IPTG,a 54 kD recombinant protein was expressed and separated by SDS-PAGE electrophoresis.The research provides a base for further studying on the protein mechanism and the potential applications of sorbitol-6phosphate dehydrogenase. |
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| Keywords: | apple sorbitol sorbitol-6-phosphate dehydrogenase prokaryotic expression |
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