| Effects of the Combined Use of Benazepril and Valsartan on Apoptosis in the Kidney of Rats with Adriamycin-induced Nephritic Gomerulosclerosis |
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| 引用本文： | 韩子明,邢燕,王宏伟,梁秀玲,周建华. Effects of the Combined Use of Benazepril and Valsartan on Apoptosis in the Kidney of Rats with Adriamycin-induced Nephritic Gomerulosclerosis[J]. 华中科技大学学报(医学英德文版), 2004, 24(3): 254-258. DOI: 10.1007/BF02832005 |
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| 作者姓名： | 韩子明 邢燕 王宏伟 梁秀玲 周建华 |
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| 作者单位： | [1]DepartmentofPediatrics,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030,China [2]DepartmentofPediatrics,theFirstAffiliatedHospitalofXinxiangMedicalCollege,Xinxiang453100,China |
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| 摘 要： | The effects of the combined use of angiotensin converting enzyme inhibitor (ACEI) benazepril and angiotensin 11 type 1 receptor antagonist (AT1RA) valsartan on apoptosis and the expression of apoptosis-related proteins Fas and FasL in the kidney of rats with adriamycin-induced nephritic glomerulosclerosis was investigated. Uninephrectomy and the injection of adriamycin induced the rat model of glomerulosclerosis. Benazepril (6 mg/kg), valsantan (20 mg/kg), or benazepril (3 mg/kg) plus valsantan (20 mg/kg) was respectively delivered daily by gavage to the rats in three treatment groups for 12 weeks. Apoptosis was examined by means of terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL). Immunohistochemistry was adopted to detect the expression of Fas and FasL. Software of pathological analysis quantitated the levels of Fas and FasL. The results showed that as compared with those in the control group, the kidneys in the model group had more severe glomerulosclerosis, much more apoptotic cells and higher levels of expression of Fas and FasL. The degree of glomerulosclerosis, the number of apoptotic cells and the levels of expression of Fas and FasL were reduced by benazepril and valsartan. The combined use of benazepril and valsartan had the best therapeutic effect. It was concluded that benazepril and valsartan could suppress the excessive apoptosis of kidney cells by lowering the expression of the apoptosis-related proteins Fas and FasL, so as to postpone the process of glomerulosclerosis. The combined use of benazeoril and valsartan has better theraoeutic effect.
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| 关 键 词： | 肾功能衰竭 老鼠 阿霉素 药物作用 肾血管球硬化症 病理作用 贝那普利 缬沙坦 |
| 收稿时间： | 2004-04-15 |
Effects of the combined use of benazepril and valsartan on apoptosis in the kidney of rats with adriamycin-induced nephritic glomerulosclerosis |
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| Han Ziming,Xing Yan,Wang Hongwei,Liang Xiuling,Zhou Jianhua. Effects of the combined use of benazepril and valsartan on apoptosis in the kidney of rats with adriamycin-induced nephritic glomerulosclerosis[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2004, 24(3): 254-258. DOI: 10.1007/BF02832005 |
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| Authors: | Han Ziming Xing Yan Wang Hongwei Liang Xiuling Zhou Jianhua |
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| Affiliation: | 1. Department of Pediatrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
2. Department of Pediatrics,the First Affiliated Hospital of Xinxiang Medical College,Xinxiang 455100,China |
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| Abstract: | Summary The effects of the combined use of angiotensin converting enzyme inhibitor (ACEI) benazepril and angiotensin II type 1 receptor antagonist (AT1RA) valsartan on apoptosis and the expression of apoptosis-related proteins Fas and FasL in the kidney of rats with adriamycin-induced nephritic glomerulosclerosis was investigated. Uninephrectomy and the injection of adriamycin induced the rat model of glomerulosclerosis. Benazepril (6 mg/kg), valsantan (20 mg/kg), or benazepril (3 mg/kg) plus valsantan (20 mg/kg) was respectively delivered daily by gavage to the rats in three treatment groups for 12 weeks. Apoptosis was examined by means of terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL). Immunohistochemistry was adopted to detect the expression of Fas and FasL. Software of pathological analysis quantitated the levels of Fas and FasL. The results showed that as compared with those in the control group, the kidneys in the model group had more severe glomerulosclerosis, much more apoptotic cells and higher levels of expression of Fas and FasL. The degree of glomerulosclerosis, the number of apoptotic cells and the levels of expression of Fas and FasL were reduced by benazepril and valsartan. The combined use of benazepril and valsartan had the best therapeutic effect. It was concluded that benazepril and valsartan could suppress the excessive apoptosis of kidney cells by lowering the expression of the apoptosis-related proteins Fas and FasL, so as to postpone the process of glomerulosclerosis. The combined use of benazepril and valsartan has better therapeutic effect. HAN Ziming, male, born in 1964, Professor |
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| Keywords: | benazepril valsartan adriamycin nephropathy apoptosis Fas FasL |
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