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| HPLC法测定肠舒片中蔷薇酸和委陵菜酸 | |
| 引用本文： | 白淑芳,刘占云,宋菲,高颖,刘岱琳,陈虹. HPLC法测定肠舒片中蔷薇酸和委陵菜酸[J]. 中成药, 2012, 34(2): 282-285 |
| 作者姓名： | 白淑芳 刘占云 宋菲 高颖 刘岱琳 陈虹 |
| 作者单位： | 1. 天津市职业与环境危害生物标志物重点实验室,天津300162;中国人民武装警察部队医学院生药教研室,天津300162 2. 中国人民武装警察部队医学院生药教研室,天津300162;沈阳药科大学中药学院辽宁沈阳110016 3. 中国人民武装警察部队医学院生药教研室,天津,300162 |
| 基金项目： | 天津市自然科学基金项目(10JCYBJC14600) |
| 摘 要： | 目的 建立肠舒片(固公果根)中蔷薇酸和委陵菜酸的测定方法.方法 采用HPLC法测定,色谱柱为Phenomenex Luna Su C18柱；流动相为水-甲醇(32∶68)；体积流量1.0 mL/min;检测波长204 nm；柱温30℃；进样量20 μL.结果蔷薇酸和委陵菜酸的标准曲线回归方程分别为:Y=6 ×106X+75 636(R2=0.9999),Y=6×106X+ 510 504(R2=0.9999),蔷薇酸和委陵菜酸分别在8.24～82.4 μg和16.0～160.0 μg范围内线性关系良好,蔷薇酸和委陵菜酸的平均回收率分别为103.1％、100.9％.不同批次肠舒片中蔷薇酸质量分数范围为0.254～0.312 mg/g,委陵菜酸质量分数范围为0.572～0.663 mg/g.结论 所用方法简便、准确,可用于不同批次肠舒片的质量控制. |
| 关 键 词： | HPLC 肠舒片 蔷薇酸 委陵菜酸 |
Quantitation of euscaphic acid and tormertic acid in Changshu Tablet by HPLC |
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| BAI Shu-fang , LIU Zhan-yun , SONG Fei , GAO Ying , LIU Dai-lin , CHEN Hong. Quantitation of euscaphic acid and tormertic acid in Changshu Tablet by HPLC[J]. Chinese Traditional Patent Medicine, 2012, 34(2): 282-285 | |
| Authors: | BAI Shu-fang LIU Zhan-yun SONG Fei GAO Ying LIU Dai-lin CHEN Hong |
| Affiliation: | 1,2(1.Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard,Tianjin 300162,China;2.Department of Pharmacognosy,Medical College of Chinese People’s Armed Police Forces,Tianjin 300162,China;3.School of Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China) |
| Abstract: | AIM To determine the contents of euscaphic acid and tormertic acid in Changshu Tablet(Rosa odorata Sweet var.gigantea Rehd.et Wils.) by HPLC.METHODS Determination was performed on Phenomenex Luna Su C18 by HPLC and a mobile phase consisting of water-methanol(32∶ 68),with a velocity of 1.0 mL/min,column temperature was maintained at 30 ℃.The UV detector wavelength was set at 204 nm.RESULTS The linear range of euscaphic acid was between 8.24 and 82.4 μg with the average recovery of 103.1% and the regression equation Y=6×106X+75 636(R2=0.999 9).The linear range of tormertic acid was between 16.0 and 160.0 μg with the average recovery of 100.9% and the regression equation Y=6×106X+510 504(R2=0.999 9).The contents of euscaphic acid and tormertic acid in Changshu Tablet were in the ranges of 0.254-0.312 mg/g and 0.572-0.663 mg/g.CONCLUSION The method is simple and reliable,and can be used for the quality control of Changshu Tablet. |
| Keywords: | HPLC Changshu Tablet euscaphic acid tormertic acid |
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