子宫腺肌病中细胞凋亡和增殖状况及其相关基因表达研究

目的:探讨子宫腺肌病组织中细胞凋亡和增殖状况,及其相关调控基因survivin和PCNA在其中表达的意义。方法:应用免疫组化SP法,检测了30例子宫腺肌病患者的在位和异位子宫内膜及25例正常子宫内膜组织中survivin和PCNA的表达。结果:survivin蛋白在正常子宫内膜及腺肌病在位内膜分泌晚期的腺上皮细胞内有表达,而在增殖期无表达。在子宫腺肌病月经各期的异位内膜中,survivin蛋白持续表达,无周期性变化,其表达高于在位内膜及对照组内膜差异有显著性(P<0.05)。PCNA组织学评分在子宫腺肌病异位内膜,在位内膜以及对照组内膜,三组腺上皮细胞间有显著差异(P<0.05)。结论:腺肌病子宫内膜细胞凋亡减弱的同时增殖能力增强,可能是子宫腺肌病的发病机理之一。

Study on apoptosis,cellular proliferation and expression of regulating genes in adenomyosis

Objective To investigate the significance of apoptosis, proliferation and expression of regulating genes in adenomyosis. Methods Expression of survivin protein and PCNA protein were detected by immunohistochemistry staining, in humans ectopic and eutopic endometrial cells (from 30 women with adenomyosis) and eutopic endometrial cells (from 25 women without adenomyosis). Results Survivin protein expression in eutopic endometrium of adenomyosis and in control group was the strongest in glandular epithelial cells during the late secretory phase, and was not stained in the proliferative phase. In ectopic endometrium of adenomyosis survivin expression was higher compared with secretory phase in control(P<0. 05),and no cyclic change was demonstrated. PCNA expression in eutopic endometrium of adenomyosis was stronger than that in control group,and was lower than that in ectopic endometrium of adenomyosis(P<0. 05). Conclusion The apoptosis is inhibited while proliferation is improved, which provides good conditions for the ectopic endometrium to invasion, plant and growth.