反相高效液相色谱法同时测定咸鸭蛋黄中的斑蝥黄和苏丹红

采用反相高效液相色谱法同时测定了咸鸭蛋黄中的斑蝥黄和苏丹红。以乙腈-甲醇-氯仿(体积比为1∶0.5∶0.5)混合溶液提取咸鸭蛋黄中的斑蝥黄和苏丹红。提取液经减压蒸馏至近干,用乙腈定容。以乙腈-水(体积比为95∶5)为流动相,经XDB-Ｃ18色谱柱(250 mm×4.6 mm)分离,采用478 nm～520 nm~471 nm三段变波长检测,获得了较好的分离效果和较低的检测限。将该法用于咸鸭蛋黄中斑蝥黄和苏丹红的测定,苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ和斑蝥黄的回收率分别为97.34%，89.56%，90.98%，93.63%和95.15%;相对标准偏差分别为2.7%，4.3%，5.1%，4.9%和3.1%。该法简便、快速、准确。

Simultaneous Determination of Canthaxanthin and Sudan Red in Salted Duck Egg Yolk by Reversed-Phase High Performance Liquid Chromatography

A reversed-phase high performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of canthaxanthin, Sudan Red I , Sudan Red II , Sudan Red III and Sudan Red IV in salted duck egg yolk has been developed. These canthaxanthin and Sudan Red dyes were extracted with a mixture of acetonitrile, methanol, and chloroform (1 : 0.5 : 0.5, v/v). The extract was dried by a rotary evaporator. The residue was transferred to a graduated tube and diluted to 10 mL with acetonitrile. The canthaxanthin and Sudan Red dyes were separated on a XDB-C18 column (250 mm x 4.6 mm), with acetonitrile-water (95:5, v/v) as mobile phase. They were then detected at 478 nm - 520 nm - 471 nm at different retention times by UV/Vis spectrophotometer. The recoveries of sudan I , sudan II, sudan II, sudan IV, and canthaxanthin were 97.34%, 89.56%, 90.98%, 93.63% and 95.15% respectively. The relative standard deviations (RSDs) were 4.7%, 4.3%, 5.1%, 4.9% and 3.1%, respectively. The method is simple, rapid and accurate.