| Experimental Study on the Cryopreservation of LLC-PK1 Epithelial Cells with Hypoxic UW Solution |
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| 作者姓名： | 万赤丹 王春友 刘涛 王宏博 杨智勇 |
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| 作者单位： | Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China |
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| 摘 要： | The effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells （LLC-PK1 cells） were cryopreserved in hypoxic UW solution （Ar-UW group） or standard UW solution （UW group） at 4℃ for 48 h. Trypan blue staining and LDH detection were performed to evaluate the injury of the cells. The results showed that the oxygen partial pressure in Ar-UW group was significantly declined from 242±6 mmHg to 83±10 mmHg. After cryopreservation at 4℃ for 48 h, LDH leakage rate and Trypan blue-stained rate in Ar-UW group were （11.3±3.4）% and （10.5±4.7）%, respectively, which were significantly lower than in UW group （49.5±6.9）% and （47.6±9.3）% respectively, both P〈0.01]. It was concluded that lower oxygen partial pressure of UW solution was more beneficial to the cryopreservation of LLC.
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| 关 键 词： | 冷冻保存 上皮细胞 器官保存 含氧量 |
| 收稿时间： | 23 October 2006 |
| 修稿时间： | 2006-10-23 |
Experimental study on the cryopreservation of LLC-PK1 epithelial cells with hypoxic UW solution |
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| Wan?Chidan,Wang?Chunyou,Liu?Tao,Wang?Hongbo,Yang?Zhiyong.Experimental Study on the Cryopreservation of LLC-PK1 Epithelial Cells with Hypoxic UW Solution[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(4):426-428. |
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| Authors: | Wan Chidan Wang Chunyou Liu Tao Wang Hongbo Yang Zhiyong |
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| Affiliation: | Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Summary The effects of oxygen partial pressure on cryopreservation of the cells with organ preservation solution were explored. Hypoxic
UW solution was made by purging the UW solution with argon. The pig proximal tubule epithelial cells (LLC-PK1 cells) were
cryopreserved in hypoxic UW solution (Ar-UW group) or standard UW solution (UW group) at 4°C for 48 h. Trypan blue staining
and LDH detection were performed to evaluate the injury of the cells. The results showed that the oxygen partial pressure
in Ar-UW group was significantly declined from 242±6 mmHg to 83±10 mmHg. After cryopreservation at 4°C for 48 h, LDH leakage
rate and Trypan blue-stained rate in Ar-UW group were (11.3±3.4)% and (10.5±4.7)%, respectively, which were significantly
lower than in UW group (49.5±6.9)% and (47.6±9.3)% respectively, both P<0.01]. It was concluded that lower oxygen partial pressure of UW solution was more beneficial to the cryopreservation of
LLC.
WAN Chidan, male, born in 1969, Associate Professor, M.D., Ph.D. |
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| Keywords: | organ preservation solution oxygen partial pressure cryopreservation |
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