PEGDA水凝胶的光固化成形工艺及其性能评价

采用光固化成形技术制备三维结构软骨组织工程聚乙二醇双丙烯酸酯(PEGDA)水凝胶,并对其成形工艺参数进行对比研究.对聚乙二醇(400)双丙烯酸酯(PEG(400)DA)溶液光固化成形单条线及单层面的形貌尺寸进行测量,研究PEG(400) DA体积分数、激光扫描速度以及扫描线间距等参数对测量结果影响.对制备的PEGDA水凝胶成形尺寸、成分、吸水率、压缩弹性模量等进行评价,结果表明:随着曝光量以及φ(PEG(400) DA)的增加,水凝胶单条线的固化厚度与固化宽度均增加；随着激光扫描填充向量间距的增加,水凝胶单层面的固化厚度减小；随着φ(PEG(400) DA)的增加,固化后的水凝胶吸水率降低,压缩弹性模量增大.采用φ( PEG(400) DA)为30％的溶液,激光器功率为0.1W,扫描速度为1 800 mm/s,填充向量间距为0.15 mm,能够制备出定制化膝关节水凝胶软骨支架,其吸水率为191.67％,平均压缩弹性模量为0.75 MPa,满足软骨组织工程的需求.

Fabrication and Evaluation of PEGDA Hydrogel by Stereo-Lithography for Cartilage Tissue Engineering

The stereo-lithography technique for fabricating biocompatible polyethyleneglycol diacrylate(PEGDA) hydrogel with a controlled accurate architecture is developed for cartilage tissue engineering application,and the different laser scanning parameters for single-line hydrogel or 2-D layer hydrogel are comparatively investigated,The cure parameters of the single-line and 2-D layer of hydrogel scaffold are affected by PEG(400)DA content of the solution,the laser scanning speed and hatch space.Hydrogel scaffold properties,such as structure accuracy,composition,and water absorption and compression modulus are experimentally confirmed.The results showed that: Cure depth and width increase positively with the energy dosage of laser and the PEG(400)DA content of solution;Cure depth scales inversely with the hatch space;Water absorption decreases and the compression modulus increases as the PEG(400)DA content of solution varies from 30% to 60%.The optimal laser process parameters include: 0.1 W laser power,1 800 mm/s laser scanning speed,0.15 mm hatch space and 30% content of φ(PEG(400)DA) solution,customized hydrogel scaffold of knee cartilage was obtained with 191.67% water absorption rate and 0.75 MPa average compression modulus.The stereo-lithographic PEGDA hydrogel technique perfectly meets the requirements for cartilage tissue engineering.