醛固酮瘤中醛固酮合成相关酶与相应调节基因表达的变化

目的 比较醛固酮瘤(APA)与正常肾上腺组织的基因表达谱,探讨醛固酮合成过程的差异与可能的调节异常.方法 以术后病理学检查确诊为APA的组织标本(APA组,n=10)及正常肾上腺组织(对照组,n=7)作为研究对象.提取两种组织的总RNA,合成生物素标记的探针cRNA,与载有一组靶基因的基因表达谱芯片杂交;通过扫描荧光强度,计算机软件分析,得到两种组织的表达差异基因;对异常表达靶基因进行Real-time PCR验证.结果 与对照组相比,APA组有97个基因表达上调,168个基因表达下调;醛固酮合成路径中,仅CYP11B2表达显著上调;生理性调节醛固酮合成的因子中,CYB5A、CYP17A1、DUSP1、HMGCR表达下调,RENBP和NR1H2表达上调;皮质醇合成关键酶CYP17A1表达被抑制.结论 在APA的醛固酮合成路径相关酶与调节因子中,CYP11B2可能是关键合成酶;多数生理性调节因子被抑制,提示存在肿瘤性调节因素.

Expression of aldosterone synthesis related enzyme and associated regulatory factor genes in aldosterone producing adenoma

Objective To investigate the discrepancy of aldosterone synthesis process and potential regulation abnormality between aldosterone-producing adenoma(APA) and normal adrenal(NA) with microarray. Methods cRNA probes labelled with biotin were prepared from mRNA of APAs(APA group,n=10) or NAs(control group,n=7).The probes were hybridized with oligonucleotide microarray of target gene expression profile.Expression levels were read from the fluorescent intensity scanned.The difference of gene expression profile w...