实时荧光定量检测肝细胞肝癌SATB1 mRNA的表达及其临床意义

摘要：目的探讨肝癌中核基质结合区结合蛋白-1的表达及其临床意义。方法用TRIZOL提取102例肝癌组织及对应的癌
旁组织，并将其逆转录为cDNA。用实时荧光定量PCR方法检测肝细胞肝癌中SATB1 mRNA的表达，并分析SATB1基因
的表达程度与患者临床病理参数、术后复发与转移的关系。结果肝细胞肝癌组织中SATB1的表达是癌旁组织的3.27倍
（P<0.001），有统计学差异。癌组织中SATB1 mRNA表达的高低与肝硬化、AFP、肿瘤大小、癌栓、病理分化、TNM分级、术
后复发转移有关系（P<0.05）。与性别、年龄、乙型肝炎表面抗原、丙型肝炎抗体、肿瘤的数目、包膜无关（P>0.05）。SATB1
mRNA显著高表达组、高表达组、低表达组的复发率分别为82.68%，0，0；而三组的一年累计生存率分别为0，52.63%，
100%。结论SATB1 mRNA的表达与肝癌的复发转移有关，SATB1有望成为肝癌复发与转移的一个新的预后指标。

Expression of special AT-rich sequence-binding protein 1 mRNA in hepatocellular carcinoma and its clinical significance

Objective To investigate the expression of special AT-rich sequence binding protein 1(SATB1) mRNA in hepatocellular carcinoma(HCC) and explore its correlation to the clinicopathological features,surgical outcomes and metastasis of HCC.Methods The total RNA was extracted from 102 HCC tissues and the adjacent tissues,and the expression of SATB1 mRNA was detected using quantitative real-time PCR.The correlations of SATB1 mRNA expression to the clinicopathological features,postoperative recurrence and metastasi...