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| 快速高分离度液相色谱-串联质谱法测定番茄酱中番茄红素的含量 | |
| 引用本文： | 张军,杜平,孙立霞.快速高分离度液相色谱-串联质谱法测定番茄酱中番茄红素的含量[J].中国食物与营养,2010(6):54-56. |
| 作者姓名： | 张军 杜平 孙立霞 |
| 作者单位： | 1. 新疆农业职业技术学院,昌吉,831100 2. 新疆中基天然植物纯化高新技术研究院,乌鲁木齐,830088 3. 新疆天仁培训学校,昌吉,831100 |
| 摘 要： | 本文建立了快速高分离度液相色谱-串联质谱(RRLC-MSMS)测定番茄红素的方法,通过实验优化了的分析条件。选择m/z109为定量离子,碰撞能量20V;m/z413为定性离子,碰撞能量10V。在0.02～10.0mg/L浓度范围内,曲线方程:Y=718.79x2+5964.98x-111.64,R2=0.999998。S/N=3为定性限,检出浓度0.03mg/L;S/N=10为定量限,检出浓度0.1mg/L。用1.0mg/L的溶液连续进样6次,结果的精密度RSD=3.97%。测定方法中样品前处理简单,节省溶剂,用时短,定性、定量准确,满足检测分析的要求。 |
| 关 键 词： | 番茄红素 RRLC-MSMS |
Determination of Lycopene Content in Ketchup with RRLC-MSMS |
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| ZHANG jun,DU Ping,SUN Li-xia.Determination of Lycopene Content in Ketchup with RRLC-MSMS[J].Food and Nutrition in China,2010(6):54-56. | |
| Authors: | ZHANG jun DU Ping SUN Li-xia |
| Affiliation: | 1 Xinjiang Agricultural vocational technical college,Changji 831100;2 Xinjiang Chalkis Natural Plant high-tech Institute,Urumqi 830088;3 Xinjiang Godsoul Education ＆ trailing school,Changji 831100) |
| Abstract: | A method was developed for the determination of lycopene by rapid resolution liquid chromatography-tandem mass spectrometry （RRLC-MSMS） and the analytical method was optimized by the experiment.Quantitative ion was m/z109,collision energy was 20V,qualitative ion was m/z413 and collision energy was 10V.The concentration range was from 0.02mg/L to 10.0mg/L and curvilinear equation was Y=718.79x^2＋5964.98x-111.64,R^2=0.999998.S/N=3 was qualitative limit and concentration of detection was 0.03mg/L.S/N=10 was quantitative limit,detection concentration was 0.1mg/L,the solution which was 1.0mg/L was injected 6 times continuously,RSD of the precision was 3.97%. |
| Keywords: | RRLC-MSMS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
