周围神经缺血再灌注损伤与神经元凋亡的关系

目的 研究大鼠周围神经缺血再灌注损伤与脊髓神经元凋亡的关系和规律,为临床防治此类神经损伤提供理论依据.方法 采用无损伤动脉夹暂时夹闭大鼠髂总、髂内、髂外及股动脉,不同时间段开放恢复血流再灌注的大鼠周围神经缺血再灌注模型,取脊髓腰骶膨大处脊髓灰质组织通过流式细胞仪检测细胞凋亡率进行分析.结果 各实验组均可检测到凋亡细胞,但各组细胞凋亡率均有所不同.各缺血组神经细胞凋亡率明显高于假手术对照组(P<0.01).缺血4 h组神经细胞的凋亡率高于其他各组,与2、12 h组比较差异具有统计学意义(P<0.05).细胞凋亡率最高出现在缺血4 h再灌注6 h组,缺血4、6、8 h组再灌注72 h后出现细胞凋亡率的下降,甚至低于未灌注组.结论 周围神经缺血再灌注损伤可以引起神经元的凋亡.不同缺血与再灌注时间,神经细胞的凋亡率也不尽相同.

The relationship between ischemia-reperfusion injury of the peripheral nerves and neuronal apoptosis

Objective To investigate the relationship between ischemia-reperfusion injury of rat peripheral nerve and spinal cord neuronal apoptosis,and provide the theoretical basis for prevention and treatment of this kind of injury clinically.Methods Rat peripheral nerve ischemia-reperfusion injury model was created by temporary occlusion of the common iliac,internal illac,external iliac and femoral artery for various periods of time.Grey matter tissue of the spinal cord at the lumbosacral enlargement was harvested.Apoptosis rate was quantified using flow cytometry. Results Apoptotic cells were detected in all experimental groups,however every experiment group had different apoptosis rate.The apoptosis rates of all ischemia groups were higher than that of the sham operation group(P<0.01).The apoptosis rate of ischemia4 h group was the highest comparing to any other ischemia groups and the differences over ischemia 2 h and 12 h groups were statistically significant (P<0.05).The highest cell apoptosis rate occurred in the ischemia 4 h and repeffusion 6 h group.The cell apoptesis rates declined upon 72 h reperfusion following 4 h,6 h and 8 h of ischemia,to the extent even lower than that of the sham operation group.Conclusion Ischemia-reperfusion injury of the peripheral nerve can cause apoptosis of the neurons.Different ischemia and reperfusion intervals lead to different apoptosis rates.