应用戊二醛对百日咳抗原进行解毒的研究

本文报道应用戊二醛对百日咳保护性抗原解毒,并研究解毒条件与毒性及免疫原性的关系。在百日咳保护性抗原或无细胞百日咳菌苗的制造中,应用0.05%戊二醛(GA)于20℃作用4～6小时,再加入0.15～0.2%赖氨酸继续作用2小时,可以得到最佳解毒效果。用于制造无细胞百日咳菌苗符合菌苗检定要求,经37℃存放1个月后未见毒性逆转现象发生。

Investigation on the Detoxification of the Pertussis Protective Antigens with Glutaldhyde

The several methods of the detoxification on the protective antigens of B.pertussis were studied.It was found the relationships between the depth of deto- xification and the toxicity and immunogenicity of antigens.The toxicity of the anti- gens could not be detoxified completely in presence of more than 0.02% lysine before glutaldhyde was added.The temperature on detoxification was highly important.In 20℃,the effect of detoxification was complete but not in 4℃.The best procedure of detoxification for the antigens of B.pertussis was that the antigens were treated with glutaldhyde for 4～6 h at 20℃,then 0.15～0.2% lysine was added and reacted aga- in for 2 h at the same temperature.By using this procedure,the toxicity reversion of the acellular pertussis vaccine has not been found after kept under 37℃ for 1 month.