眼镜蛇毒细胞毒素对S180荷瘤小鼠抗肿瘤作用的研究 |
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引用本文: | 张锦宏,曾军,谢天炽,郭芝刚. 眼镜蛇毒细胞毒素对S180荷瘤小鼠抗肿瘤作用的研究[J]. 现代保健, 2012, 0(20): 1-3 |
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作者姓名: | 张锦宏 曾军 谢天炽 郭芝刚 |
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作者单位: | 广州医学院,广东广州510182 |
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基金项目: | 广东省自然基金(项目编号:9451018201003599); 广州医学院科研项目04-K-19资助 |
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摘 要: | 目的:探讨眼镜蛇毒细胞毒素(Cytotoxin,CTX)对S180荷瘤小鼠的抗肿瘤作用。方法:采用昆明小鼠S180实体瘤模型,通过腹腔注射CTX,观察CTX对S180小鼠的肿瘤重量、亚显微结构、凋亡率和pro-caspase-3表达量的变化。结果:CTX对S180肿瘤细胞的生长表现出明显抑制作用。0.2、0.4、0.8mg/kgCTX剂量组的抑瘤率分别为23.8%、54.1%、63.2%;透射电子显微镜和流式细胞仪检测显示S180肿瘤细胞发生凋亡;免疫印迹结果显示pro-caspase-3蛋白表达降低,提示caspase-3被激活。结论:CTX通过诱导细胞凋亡来抑制S180肿瘤细胞的生长。
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关 键 词: | 眼镜蛇毒 细胞毒素 S180 凋亡 caspase-3 |
The Antitumor Role of Naja Naja Atra Venom Cytotoxin in S180-bearing Mice |
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Affiliation: | ZHANG Jin-hong,ZENG Jun,XIE Tian-zhi,et al.Guangzhou Medical College,Guangzhou 510182,China |
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Abstract: | Objective:To investigate the antitumor activity of Naja naja atra venom cytotoxin in S180-bearing mice.Method:After the intraperitoneal injection of CTX in the S180-bearing Kunming mice,the changes of the tumor weight,ultrastructure,apoptosis rate and the expression of pro-caspase-3 were observed.Result:CTX showed significantly inhibition on the growth of S180 tumor cells.The inhibitory rates of CTX were 23.8 %,54.1 %,63.2 % at the dosages of 0.2,0.4,0.8 mg/kg,respectively.Transmission electron microscopy and flow cytometry showed that CTX induced S180 cell apoptosis.Immunoblotting showed that the expression of pro-caspase-3 protein was decreased,which suggest that caspase-3 protein is activated.Conclusion:CTX could inhibit the growth of S180 tumor cells by inducing cell apoptosis. |
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Keywords: | Naja naja atra venom cytotoxin S180 Apoptosis Caspase-3 |
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