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| Ad-METH1对培养的内皮细胞分泌活性的影响 | |
| 引用本文： | 宋保强,鲁开化,郭树忠,张阳,张琳西,王云雅.Ad-METH1对培养的内皮细胞分泌活性的影响[J].中国美容医学,2007,16(6):740-742. |
| 作者姓名： | 宋保强 鲁开化 郭树忠 张阳 张琳西 王云雅 |
| 作者单位： | 1. 第四军医大学西京医院全军整形外科研究所,陕西,西安,710032 2. 解放军第458医院外科,广东,广州,510602 3. 第四军医大学西京医院核医学科 |
| 摘 要： | 目的：研究基因重组血管抑制剂Ad-METH1（metalloprotease and thrombospondin1）对培养的内皮细胞分泌活性的影响,探讨血管生成抑制防治增生性瘢痕的可能机制。方法：制备重组血管抑制剂Ad-METH1,转染培养的人脐静脉内皮细胞,通过放射免疫分析法及酶联免疫分析法研究Ad-METH1对内皮细胞分泌内皮素-1（endothelin-1,ET-1）及碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）的影响。结果：基因转染后24h内皮细胞ET-1、bFGF分泌受到明显抑制。结论：Ad-METH1对内皮细胞ET-1、bFGF分泌活性有影响,此是其早期参与抑制增生性瘢痕形成的可能机制之一。 |
| 关 键 词： | 基因转染 内皮细胞 内皮素-1 碱性成纤维细胞生长因子 |
| 文章编号： | 1008-6455（2007）06-0740-03 |
| 修稿时间： | 2007-01-242007-05-14 |
The effect of Ad-METH1 on secretion of ET-1 and bFGF from endotheliocyte |
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| SONG Bao-qiang,LU Kai-hua,GUO Shu-zhong,ZHANG Yang,ZHANG Lin-xi,WANG Yun-ya.The effect of Ad-METH1 on secretion of ET-1 and bFGF from endotheliocyte[J].Chinese Journal of Aesthetic Medicine,2007,16(6):740-742. | |
| Authors: | SONG Bao-qiang LU Kai-hua GUO Shu-zhong ZHANG Yang ZHANG Lin-xi WANG Yun-ya |
| Abstract: | Objective To investigate the effect of Ad-METH1 on endotheliocyte secretion activity and approach the possible mechanism of preventing hypertrophic scar by angiogenesis inhibition. Methods Ad-METH1 was prepared and used to transfect the cultural HUVEC. Then, the concentration of ET-1 and bFGF secreted from endotheliocyte in cultural supernate were studied by radioimmunoassay(RIA) and enzyme-linked immunosorbent assay (ELISA). Results At the 24th hour after Ad-METH1 transfection, in experiment group, the concentration of ET-1 and bFGF in cultural supernate decreased markedly comparing to that in control group. Conclusion Inhibition of Ad-METH1 on secretion of ET-1 and bFGF from endotheliocyte might have act an important role in preventing the form of hypertrophic scar. |
| Keywords: | gene transfection endotheliocyte ET-1 bFGF |
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