| 刺五加总RNA提取方法的比较 |
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| 引用本文： | 吴鹏,修乐山,邢朝斌,田喜凤.刺五加总RNA提取方法的比较[J].中国园艺文摘,2013(11):7-8,59. |
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| 作者姓名： | 吴鹏 修乐山 邢朝斌 田喜凤 |
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| 作者单位： | 河北联合大学生命科学学院,河北唐山,063000 |
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| 基金项目： | 河北省自然科学基金-石药集团医药联合研究基金项目（H2012401006）；国家大学生创新创业训练计划（201310081007）；河北省大学生创新创业训练计划（201310081007）；河北联合大学大学生创新性实验计划（X2013042）。 |
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| 摘 要： | 为了获取到高质量的刺五加总RNA，以叶片为试材，比较不同提取方法的提取效果。结果表明，改良的异硫氰酸胍法效果最优，方法简单、经济，通过该方法获得的总RNA能够很好的满足后续分子生物学实验的要求，试剂盒方法效果次之，CTAB法效果第三，Trizol法最差。
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| 关 键 词： | 刺五加 总RNA 提取 |
Comparison of different total RNA extraction methods for Eleutherococcus senticosus |
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| Authors: | WU Peng XIU Le-shan XING Zhao-bin TIAN Xi-feng |
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| Affiliation: | WU Peng,XIU Le-shan,XING Zhao-bin,TIAN Xi-feng |
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| Abstract: | In order to get the high quality of total RNA of Eleutherococcus senticosus, the extraction effects of different extraction methods were compared by taking the leaf of E. senticosus as the test materials. The results indicated that the improved guanidine thiocyanate method is the best which is simple and economic. The total RNA extracted with this method can satisfy the requirements of the molecular biology of the follow-up experiments. And it is followed by the kit method. The effect of CTAB method is third, and the Trizol method is the worst. |
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| Keywords: | Eleutherococcus senticosus total RNA extraction |
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