| 凝血因子IX基因剔除小鼠的培育历史与建系方法 |
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| 引用本文： | 郝光荣,孙伟,汤球,崔淑芳,黄立志.凝血因子IX基因剔除小鼠的培育历史与建系方法[J].中国实验动物学报,2003,11(2):120-121. |
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| 作者姓名： | 郝光荣 孙伟 汤球 崔淑芳 黄立志 |
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| 作者单位： | 第二军医大学实验动物中心,上海,200433 |
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| 基金项目： | 上海市科学技术发展基金资助项目(9949190 3 2 ) |
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| 摘 要： | 目的 培育凝血因子IX基因剔除小鼠近交系。方法 采用全同胞兄妹对交配 ,每代选用第一胎小鼠留种 ,记录繁殖性能 ;第 8代开始筛选纯合子。结果 凝血因子IX基因剔除小鼠已近交培育到第 12代 ,交配年龄 70 - 90d ,平均每窝产仔数 5只以上 ,离乳数 3只以上。结论 纯合子FIX剔除基因小鼠在子代鼠中能稳定遗传
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| 关 键 词： | 因子IX 小鼠 基因敲除 培育 |
| 文章编号： | 1005-4847(2003)01-0120-02 |
| 修稿时间： | 2002年2月2日 |
Study on Breeding and Management of the Coagulation Factor IX-knockout Mouse |
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| HAO Guang rong,SUN Wei,TANG Qiu,CUI Shu fang,HUANG Li zhi.Study on Breeding and Management of the Coagulation Factor IX-knockout Mouse[J].Acta Laboratorium Animalis Scientia Sinica,2003,11(2):120-121. |
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| Authors: | HAO Guang rong SUN Wei TANG Qiu CUI Shu fang HUANG Li zhi |
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| Abstract: | Objective To establish the inbred strain of the coagulation factor IX knockout mice. Methods\ The mice were bred by brother sister first mating with reproductive performance recorded.The homogeneous mice were selected after F8 Results\ Factor IX knockout mice were developed in the 12th generation.The age of mating was 70-90 day.The average birth litter size was over 5,average weaning litter size was over 3 Conclusion\ The coagulation factor IX knockout mice can be generated steadily. |
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| Keywords: | Factor IX Mice Knockout Breeding |
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