Reduced secretion of triacylglycerol in CaCo-2 cells transfected with intestinal fatty acid-binding protein |
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Authors: | Ane Gedde-Dahl Mari Ann Kulseth Trine Ranheim Christian A. Drevon Arild C. Rustan |
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Affiliation: | (1) Department of Pharmacology, School of Pharmacy, University of Oslo, N-0316 Oslo, Norway;(2) Institute for Nutrition Research, University of Oslo, N-0316 Oslo, Norway;(3) Laboratory of Clinical Biochemistry, Haukeland Hospital, P.O. Box 1, N-5021 Bergen, Norway |
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Abstract: | The fatty acid-binding proteins are hypothesized to be involved in cellular fatty acid transport and trafficking. We established CaCo-2 cells stably transfected with intestinal fatty acid-binding protein (I-FABP) and examined how the expression of this protein may influence fatty acid metabolism. I-FABP expression was detectable in I-FABP-transfected cells, whereas parent CaCo-2 cells as well as mock-transfected cells failed to express detectable levels of I-FABP mRNA or protein at any stage of differentiation. For studies of lipid metabolism, cells were incubated with [14C]oleic acid in taurocholate micelles containing monoolein, and distribution of labeled fatty acid in cellular and secreted lipids was examined. In one transfected cell clone, expressing the highest level of I-FABP, labeled cellular triacylglycerol increased approximately twofold as compared to control cells. The level of intracellular triacylglycerol in two other I-FABP-transfected clones resembled that of control cells. However, secretion of triacylglycerol was markedly reduced in all the I-FABP-expressing cell lines. Our data suggest that increased expression of I-FABP leads to reduced triacylglycerol secretion in intestinal cells. |
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