利用苹果SSR引物分析山楂属植物遗传关系

SSR引物在不同物种间具有通用性,从141对苹果属(Malus spp.)SSR引物中筛选出10对适合于山楂属(Crataegus spp.)植物的SSR引物,并对8个种37份山楂种质资源的遗传关系进行了分析。10对SSR引物共检测到91个多态性谱带,每个位点的等位基因数为3～13个,平均为9.1个。位点杂合度为0.432～0.790,平均为0.688。10对SSR引物可以将20份山楂资源区分开,17份不能区分的资源分为3组,第1组为3个伏山楂品种,第2组和第3组分别包括大果山楂的2个和12个品种。基于SSR标记构建的聚类树状图将供试37份山楂资源分成2个类群,第1类群包括6个山楂野生种,第2类群包括供试的所有伏山楂、山楂和大果山楂资源。该聚类结果与传统形态学分类一致。

Assessment of genetic relationship in Crataegus genus by the apple SSR primers

The transferability of SSRs information from one species to another species has been proven. In this study, we attempted to transfer 141 SSR primers derived from apple to hawthorn, and study the genetic relationship among 37 hawthorn germplasms, belonging to 8 species. Only 10 primer pairs which successfully amplified clear and polymorphic band patterns indicating single locus were selected for hawthorn genetic analysis. A total of 91 alleles were detected from 10 SSR loci, with an average of 9.1 alleles from 3 to 13. Heterozygosity for individual locus ranged from 0.432 to 0.790, with an average heterozygosity for 10 loci equaling 0.688. Twenty accessions could be differentiated from each other. seventeen accessions that could not be distinguished were divided into 3 groups: the first group included 3 genotypes of Crataegus brettschneideri; the second and the third group included 2 and 12 cultivars of C. pinnatifida var. major respectively. UPGMA cluster analysis of the 37 hawthorn accessions based on SSR markers resulted in 2 clusters which appeared to be related to traditional morphology classification. Cluster I contains 6 wild species of hawthorn, and the accessions of C. brettschneideri, C. pinnatifida and C. pinnatifida var. major grouped in cluster II.