白花丹参中脂溶性成分提取工艺的优选及HPLC法含量测定

目的优选白花丹参中脂溶性成分的最佳提取工艺。方法实验中采用超声提取技术对白花丹参根进行处理,以正交实验法来考察不同因素对超声提取液中脂溶性成分含量的影响并以高效液相色谱法进行的含量测定为考察指标优选出白花丹参中脂溶性成分的最佳提取工艺。结果白花丹参中脂溶性成分最佳提取工艺为:A1B2C3D1即10倍量75%的无水乙醇浸泡6h,超声30min,抽滤,超声1次。丹参酮ⅡA在7.5~50μg.ml-1范围内线性关系良好。结论超声技术操作简便快速,试验重复性好,结果满意,在丹参酮的提取中可适当推广使用。

Optimizing extraction technique of liposoluble constituent in Baihua Danshen and determining it by HPLC method

OBJECTIVE Optimizing extraction craft of liposoluble constituent in Baihua Danshen.METHODS Ultrasonic extractive technique was used to treat the material.Orthogonal test was also used to research the affect factors of the liposoluble componens in Baihua Danshen.The extraction yields of Tanshinone ⅡA were determined by HPLC.RESULTS The best extracting technique was A_1B_2C_3D_1,10 times of 75% ethyl alcohol soaked for 6 hours,supersonic 30 minutes,sucking filtration and then supersonic one time.CONCLUSION Supersonic technical operation was simple,fast and had good reproducibility,result was satisfied.May promote the use suitably for salvia miltiorrhiza′s extraction.