血虚证小鼠模型骨髓细胞红系祖细胞、粒单系祖细胞变化规律及中药复方对其的影响

目的：研究血虚证小鼠模型骨髓造血祖细胞体外增殖水平及变化规律，以及中药复方地甘口服液，红霖四物口服液对其的影响。方法：以Balb/c小鼠为实验对象，分别采用免疫介导法和综合放血法制作血虚证模型。采集其骨髓有核细胞进行培养；并分组灌服地甘口服液，红霖四物口服液，比较观察各组小鼠骨髓细胞体外培养CFU－E，BFU－E，CFU－GM集落的形成情况。结果：两种血虚证小鼠模型的CFU－E，BFU－E，CFU－GM集落形成较空白对照组显下降，尤以前为甚；两种中药复方均可促进模型小鼠骨髓细胞的增殖，但对于免疫介导血虚证模型，两治疗组的效果存在一定差异。结论：血虚证小鼠模型骨髓造血干（红9系，粒单系）细胞的体外增殖较正常小鼠明显下降，而地甘口服液，红霖四物口服液可以促进骨髓CFU－E，BFU－E，CFU－GM集落的形成。

The Changing Regularities of Blood-Deficiency-Syndrome on Hematopoietic Stem Cells (CFU-E,BFU-E,CFU-GM)and the Effects of Chinese Herbal Compound Formulas on Them in Mice

To study the changing regularities of Blood Deficiency Syndrome(BDS)on CFU E?BFU E?CFU GM of myeloid cells in mice and the effects of Chinese herbal compound formulas,Digan oral liquid(DGOL) and Siwu oral liquid(SWOL),on the models.Methods:Balb/c mouse were selected as the experimental objects.The models of BDS were made by immunologically mediation and compound bleeding,which were treated by DGOL and SWOL simultaneously.After that,the femora were taken and the bone myeloid cell(BMC)suspension were cultured.The colony forming units of CFU E?BFU E and CFU GM of varied groups were observed and compared.Results: The colony forming units of CFU E,BFU E and CFU GM of two models of BDS were lower than that of control group,especially the immunologically mediated group.The two Chinese herbal compound formulas could enhance the units formed,but the effects of them had a little difference.Conclusion: The proliferation of bone hematopoietic stem cells of the models of BDS was lower than that of normal mice.The DGOL and SWOL can enhance the proliferative function of them.