柱晶白霉素产生菌的诱变育种和发酵研究

柱晶白霉素产生菌经常规诱变及原生质体多次再生处理，获菌株９１－９１１，其产量比原始菌株提高了３５倍以上；清除了原菌株的菌丝毒性；主组分Ａ５含量也有明显增加。为充分发挥９１－９１１菌株的生产潜力，采用均匀设计方法对发酵培养基进行优化，获得ｂ培养基。９１－９１１菌株用６培养基在２００Ｌ、１０ｔ规模发酵罐上试验，证实９１－９１１菌株确是一株高产稳产的优良生产菌种。

STUDY ON THE MUTANTION BREEDING AND FERMENTATION OF LEUCOMYCIN PRODUCER

Leucomycin high producer 91-911 was obtained by conv- entional mutantion breeding and protoplast regeneration.As a result, theproduction of strain 91-911 was increased by more than 35-foldl the toxinof mycelium of original strain was eliminated;the active component Leu-comycin A5 was highly increased.The well-distributed design method was adopted to select the optimum fermentation medium for increasing productivity of strain 91-911.With the optimum medium obtainded,train 91-911 was investigated in 200 liter and 10 ton fermentor.It was proved that strain 91- 911 is a high and stable producing strain for industrial production.