过度训练对大鼠心肌线粒体膜和基质游离钙的影响

采用模拟过度训练大鼠模型，观察了大鼠心肌线粒体的MDA、巯基（-SH）、还原型谷胱苷肽（GSH）、磷脂A2（PLA2）、游离钙（Fca2＋）等指标，结果表明：过度训练组大鼠心肌线粒体与对照组相比，-SH含量和GSH含量显著降低（P〈0．01），PLA2活性显著增加（P〈0．01）；MDA、Fca2＋含量明显升高（P〈0．01）．本文根据以上结果探讨了运动时机体产生的活性氧的氧化过程与过度训练后心肌线粒体膜结构变化以及游离钙之间的关系，提出过度训练后PLA2活性增加可能是其发生机制的关键指标，通过对这一指标的进一步观察能更好地了解过度训练发生的分子机制。

Effects of Overstraining on Myocardial Mitochondria Membrane and Free Calcium of Matrix Rat

Rats on overtraining were used as experimental models. Sulfhydryl (-SH), Glutathione(GSH), phosphlipasdA2(PLA2) and free Calcium(Ca2+) of myocardial mitochondrion after overtraining in rats were investigated.The results showed that-SH and GSH in myocardial mitochondrion after overtraining were significantly degraded than that of the control group;and Ca2+and MDA content of myocardial mitochondria matrix were significantly increase. According to the results, the relationship among the oxidant reduction reaction of exercise induced reactive oxygen species, the changes of myocardial mitochondria membrane structure and the free calcium of matrix was discussed.It is suggested that increasing of PLA2 activity might be a key parameter of the changes of myocardial mitochondria membrane structure and function after overtraining.By means of further observation the molecules mechanism of overtraining appearance could be further deeply understood.;