| PEI基因载体靶向遮蔽体系的制备和表征 |
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| 引用本文： | 林琳,田华雨,夏加亮,焦自学,董璇,陈学思.PEI基因载体靶向遮蔽体系的制备和表征[J].中国科学:化学,2011(2):368-373. |
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| 作者姓名： | 林琳 田华雨 夏加亮 焦自学 董璇 陈学思 |
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| 作者单位： | 中国科学院长春应用化学研究所生态环境高分子材料重点实验室;中国科学院研究生院; |
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| 基金项目： | 国家自然科学基金(50873102,50903009,21074129,50733003),国家自然科学基金-A3前瞻计划(20621140369); 吉林省科技发展项目(20090128,20096018) |
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| 摘 要： | 将RGD短肽接枝到聚谷氨酸(PGA)上,制备了一种靶向性的基因载体遮蔽材料PGA-RGD.通过凝胶电泳实验及体外转染实验证明得出RGD的引入增加了载体材料与细胞表面受体的特异性作用,在载体表面正电荷得到遮蔽的同时,转染效率还得到了一定程度的增加.同时,对转染了48h的三元复合物进行MTT细胞毒性测试表明,PGA遮蔽的基因载体体系(PGA/PEI/DNA)和PGA-RGD遮蔽的基因载体体系(PGA-RGD/PEI/DNA)的细胞毒性均低于PEI/DNA复合物体系.本文开发的基因载体改性方法不仅可以对复合物颗粒表面的正电荷进行遮蔽,从而降低复合物体系对非目标组织的非特性异作用;同时引入的RGD靶向短肽还可以提高载体的靶向性,这一改性策略对推动阳离子聚合物基因载体在体内的应用具有重要意义.
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| 关 键 词： | PEI 基因载体 遮蔽体系 靶向作用 聚谷氨酸 |
Synthesis and characterization of targeting shielding system for PEI gene carrier |
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| LIN Lin,TIAN HuaYu,XIA JiaLiang,JIAO ZiXue,DONG Xuan, & CHEN XueSi Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Science,Changchun ,China Graduate University of Chinese Academy of Sciences,Beijing ,China.Synthesis and characterization of targeting shielding system for PEI gene carrier[J].Scientia Sinica Chimica,2011(2):368-373. |
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| Authors: | LIN Lin TIAN HuaYu XIA JiaLiang JIAO ZiXue DONG Xuan & CHEN XueSi Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied Chemistry Chinese Academy of Science Changchun China Graduate University of Chinese Academy of Sciences Beijing China |
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| Affiliation: | LIN Lin1,TIAN HuaYu1,XIA JiaLiang1,2,JIAO ZiXue1,DONG Xuan1,2 & CHEN XueSi1 1 Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Science,Changchun 130022,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China |
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| Abstract: | RGD peptides were grafted to the carboxyl groups of poly(glutamic acid) to get a targeting shielding carrier(PGA-RGD) for polyethyleneimine(PEI) gene carrier.The experiment results showed that PGA-RGD can effectively shield PEI/DNA complex particles.Gel retardation assay showed that DNA can not be released by PGA-RGD from complexes under experimental conditions for gene transfection.For PGA/PEI/DNA system,its gene transfection efficiency obviously decreased because PGA shielding carrier coating on PEI/DNA c... |
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| Keywords: | polyethyleneimine(PEI) gene carrier shielding system targeting poly(glutamic acid) |
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