| 超高效液相色谱-串联质谱法检测河鲀毒素 |
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| 引用本文： | 王丽英,任贝贝,杨立新,刘印平,常凤启,路杨.超高效液相色谱-串联质谱法检测河鲀毒素[J].食品安全质量检测技术,2015,6(10):3822-3826. |
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| 作者姓名： | 王丽英 任贝贝 杨立新 刘印平 常凤启 路杨 |
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| 作者单位： | 河北省疾病预防控制中心,河北省疾病预防控制中心,河北省疾病预防控制中心,河北省疾病预防控制中心,河北省疾病预防控制中心,河北省疾病预防控制中心 |
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| 摘 要： | 目的建立准确、快速检测河鲀毒素(tetrodotoxin,TTX)的超高效液相色谱-串联质谱(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)的分析方法,了解市售烤鱼片、鱿鱼干制品和鱼丸中河鲀毒素污染状况。方法样品经2%乙酸/甲醇溶液超声离心提取后,通过MCX固相萃取柱进行净化处理,采用液相色谱串联质谱法进行检测。结果市售的107份样品中,鱿鱼干制品和鱼丸中没有检出TTX;64份烤鱼片样品中检出16份含有TTX,检出率为25.0%。结论部分烤鱼片中TTX呈阳性,建议生产厂家严把原料关,监管部门应加强对市售烤鱼片标签标识的监管。
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| 关 键 词： | 河鲀毒素 烤鱼片 鱿鱼干制品 鱼丸 液相色谱串联质谱法 |
| 收稿时间： | 2015/8/10 0:00:00 |
| 修稿时间： | 9/8/2015 12:00:00 AM |
Determination of tetrodotoxin by ultra performance liquid chromatogra-phy-tandem mass spectrometry |
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| WANG Li-Ying,REN Bei-Bei,YANG Li-Xin,LIU Yin-Ping,CHANG Feng-Qi and LU Yang.Determination of tetrodotoxin by ultra performance liquid chromatogra-phy-tandem mass spectrometry[J].Food Safety and Quality Detection Technology,2015,6(10):3822-3826. |
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| Authors: | WANG Li-Ying REN Bei-Bei YANG Li-Xin LIU Yin-Ping CHANG Feng-Qi and LU Yang |
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| Affiliation: | Hebei Provincial Center for Disease Control and Prevention,Hebei Provincial Center for Disease Control and Prevention,Hebei Provincial Center for Disease Control and Prevention,Hebei Provincial Center for Disease Control and Prevention,Hebei Provincial Center for Disease Control and Prevention,Hebei Provincial Center for Disease Control and Prevention |
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| Abstract: | Objective To establish an analysis method for detecting tetrodotoxin (TTX) by using high performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) and to elucidate the contamination situation of TTX in grilled fillet, shredded squidand and fish ball. Methods TTX was extracted from sample by 2% acetic acid- methanol, and then purified by solid-phase extraction with MCX. The samples were analyzed by liquid chromatography tandem mass spectrometry. Results TTX was detected in shredded squidand and fish ball. 16 grilled fillet samples were detected TTX and the detection rate was 25.0%. Conclusion TTX was detected in some grilled fillet. The raw materials should strict control by manufacturers and the grilled fillet product should increase regulation by the regulatory authorities. |
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| Keywords: | tetrodotoxin grilled fillet shredded squidand fish ball high performance liquid chromatography-tandem mass spectrometry |
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