浓缩~(235)U诱发免疫细胞凋亡的电镜和琼脂糖凝胶电泳研究

研究了浓缩235U内照射诱发人淋巴细胞白血病细胞株Molt－4细胞和巨噬细胞株Ana－1细胞的细胞调亡。估算了浓缩235U在不同阶段培养细胞中的辐射累积吸收剂量。通过电镜对免疫细胞的形态观察，发现Molt－4和Ana－1细胞在受235U内照射作用下可呈现核染质边聚，DNA链断裂和膜包裹的凋亡小体形成。对Molt－4和Ana－1细胞的DNA抽提，进行DNA琼脂糖凝胶电泳也显示出阶梯状条带形成的细胞调亡特征。从而证实了浓缩235U内照射可导致免疫细胞Molt－4和Ana－1细胞的凋亡发生。

Electron microscopic and agarose gel electrophoretic studies on apoptosis in immune cells induced by enriched ~(235)U

At present, the apoptosis in Molt-4 cell(a humaan acute lymphoblastic leukemiacell line) and Ana-1 cell (a macrophage cell line) were studied after internalirradiation with enriched 235U. The cumulative radiation absorption dose of 235U incultural cells through different periods were estimated, The morphological changes,which observed by electron microscope, indicated that Molt-4 and Ana-1 immunecells after incubation with 235U, displayed nuclear fragmentation, margination ofcondensed chromatin, as well as the membrane-bounded apoptotic bodies formation.The agarose gel electrophoretic observations showed the DNA ladder paternformation in Molt-4 cell as well as in Ana-1 cell. The experimental results showedthat apoptosis induced by 235U in immune cells, were dependent on the 235U-treatedtime and cumulative radiation absorption dose.