| 活性乳酸菌饮料污染菌总数检测方法 |
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| 引用本文： | 马德良,蔡芷荷,吴清平.活性乳酸菌饮料污染菌总数检测方法[J].中国乳品工业,2010,38(4). |
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| 作者姓名： | 马德良 蔡芷荷 吴清平 |
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| 作者单位： | 1. 广东环凯微生物科技有限公司,广州,510700
2. 广东省微生物研究所,广州,510700 |
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| 摘 要： | 在培养基中添加无菌TTC(三苯基四氮唑),最终质量浓度为2.5mg/L,使菌落变红,方便观察计数.发现使用营养琼脂倾注法检验,至10-4稀释度才能消除样品中营养成分的干扰;而采用滤膜法,能有效消除饮料中营养成分的干扰,可以准确、灵敏地检验活性乳酸菌饮料中污染菌数,检测下限达到10mL-1.整个货架期,蜡样芽孢杆菌、大肠埃希氏菌、沙门氏菌等污染菌在活性乳酸菌饮料中可以存活,建立准确简便的活性乳酸菌饮料菌落总数检测方法非常必要.
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| 关 键 词： | 乳酸菌饮料 污染菌总数 检测培养基 检测方法 |
Detection method of the total contaminated bacteria for the active Lactobacillus beverage |
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| MA De-liang,CAI Zhi-he,WU Qing-ping.Detection method of the total contaminated bacteria for the active Lactobacillus beverage[J].China Dairy Industry,2010,38(4). |
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| Authors: | MA De-liang CAI Zhi-he WU Qing-ping |
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| Affiliation: | MA De-liang1,CAI Zhi-he1,WU Qing-ping2(1.Guangdong Huankai Microorganism Technology Company,Guangzhou 510700,China,2.Microorganism institute of Guangdong Province,China) |
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| Abstract: | In the present study 2.5 mg/L sterile TTC was put into the medium to make the bacteria red for the convenience counting and found that the disturbance of the nutrient compounds could be vanished till 10-4 dilution.However the filter membrane method could eliminate the dis turbance of the nutrition in the beverage to detect the contaminated bacteria accurately and effectively which detection lower limit can reach to 10 mL-1.Due to the living of the contaminated bacteria including Bacillus cereus,Escherichia ... |
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| Keywords: | Lactobacillus beverage total number of contaminated bacteria detection culture medium detection method |
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