| 腺苷A1受体拮抗剂DPCPX对低氧复氧脑星形胶质细胞影响的实验研究 |
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| 引用本文： | 王静雯.腺苷A1受体拮抗剂DPCPX对低氧复氧脑星形胶质细胞影响的实验研究[J].国际病理科学与临床杂志,2010,30(5). |
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| 作者姓名： | 王静雯 |
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| 作者单位： | 济南军区总医院病理科,济南,250031 |
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| 摘 要： | 目的:观察腺苷A1受体拮抗剂1,3-二丙基-环戊黄嘌呤(8-cyclopentyl-1,3-dipropylxanthine, DPCPX)对体外培养低氧复氧(hypoxia and reoxygenation, H/R)大鼠脑星形胶质细胞促红细胞生成素(erythropoietin, EPO)释放量、谷氨酰胺合成酶(glutamine synthase, GS)、超氧化物歧化酶(superoxide dimutase, SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-PX)活性的影响,探讨腺苷A1受体在H/R星形胶质细胞神经保护中的作用.方法:对大鼠大脑皮层星形胶质细胞进行体外培养并建立H/R模型,在低氧同时加入终浓度为100 nmol/L DPCPX 12 h后复氧1,6,12,24 h,检测EPO释放量,GS,SOD和GSH-PX活性的变化.结果:与对照组比较,DPCPX组H/R星形胶质细胞的EPO释放量显著减少;GS,SOD和GSH-PX的活性降低.结论:腺苷A1受体参与H/R星形胶质细胞的神经保护作用.
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| 关 键 词： | 腺苷A1受体 低氧 星形胶质细胞 |
Effect of adenosine A1 receptor antagonist DPCPX on cerebral astrocytes after hypoxia and reoxygenation |
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| WANG Jingwen.Effect of adenosine A1 receptor antagonist DPCPX on cerebral astrocytes after hypoxia and reoxygenation[J].Journal of International Pathology and Clinical Medicine,2010,30(5). |
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| Authors: | WANG Jingwen |
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| Affiliation: | WANG Jingwen (Department of Pathology,Jinan Military General Hospital,Jinan 250031,China) |
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| Abstract: | Objective To study the effects of adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(DPCPX) on the release of astrocytic erythropoietin (EPO),the activities of glutamine synthase (GS),superoxide dimutase (SOD) and glutathione peroxidase (GSH-PX) after hypoxia and reoxygenation (H/R) in rats,and to explore its role in the astrocytic neuroprotection.Methods Primary cultured rat cortical astrocytes and the H/R model was established.100 nmol/L DPCPX was added into media at the beginning of hypo... |
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| Keywords: | adenosine A1 receptor hypoxia astrocytes |
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