| 响应面法优化两歧双歧杆菌益生元类冻干保护剂 |
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| 引用本文： | 陈 合,齐康儒,李轶超,刘 誉,胡 曼,吴玉喜. 响应面法优化两歧双歧杆菌益生元类冻干保护剂[J]. 中国酿造, 2017, 36(5): 81. DOI: 10.11882/j.issn.0254-5071.2017.05.01７ |
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| 作者姓名： | 陈 合 齐康儒 李轶超 刘 誉 胡 曼 吴玉喜 |
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| 作者单位： | 1.陕西科技大学食品与生物工程学院，陕西西安710021；2.陕西和氏乳品有限公司，陕西宝鸡721003 |
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| 基金项目： | 陕西省科技统筹创新工程计划项目（2016KTCL02-30） |
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| 摘 要： | 以两歧双歧杆菌（Bifidobacterium bifidum）为试验菌株，以冻干存活率为评价指标，采用中心组合试验设计对两歧双歧杆菌益生元类冻干保护剂进行优化。结果显示，益生元类冻干保护剂的优化配方为菊糖13%，水苏糖11%，低聚木糖7%，经重复试验验证后的冻干存活率是（88.7±1.3）%，与预测值无显著性差异（P＞0.05），这说明采用响应面法优化两歧双歧杆菌冻干保护剂是可行的。
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| 关 键 词： | 益生元 两歧双歧杆菌 冷冻干燥 冻干保护剂 优化 |
Optimization of Bifidobacterium bifidum prebiotics cryoprotector by response surface method |
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| CHEN He,QI Kangru,LI Yichao,LIU Yu,HU Man,WU Yuxi. Optimization of Bifidobacterium bifidum prebiotics cryoprotector by response surface method[J]. China Brewing, 2017, 36(5): 81. DOI: 10.11882/j.issn.0254-5071.2017.05.01７ |
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| Authors: | CHEN He QI Kangru LI Yichao LIU Yu HU Man WU Yuxi |
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| Affiliation: | 1.School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China;
2.Shaanxi Heshi Dairy Co., Ltd., Baoji 721003, China |
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| Abstract: | Using the Bifidobacterium bifidum as the test strain and freezing survival rate as evaluation index, B. bifidum prebiotics cryoprotector was optimized by central composite design. The results showed that the optimum formula of the prebiotics cryoprotector was inulin 13%, stachyose 11%, xylooligosaccharide 7%. Under the conditions, the freezing survival rate was (88.7±1.3)% by repeated verification tests, which had no significant difference with the predicted value (P＞0.05). The study indicated that optimization of B. bifidum cryoprotector was feasible by response surface method. |
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| Keywords: | prebiotics Bifidobacterium bifidum freeze drying cryoprotector optimization |
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