柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞生长的作用机制研究

目的: 研究柚皮苷对非小细胞肺癌细胞生长的作用及机制。方法: 应用SRB法检测柚皮苷对非小细胞肺癌细胞株生长的抑制作用。用Western blot法检测柚皮苷对p70S6K的调控作用。利用质粒过表达p70S6K，用SRB法检测过表达p70S6K后对柚皮苷抑制细胞生长作用的影响。利用小干扰RNA阻抑p70S6K的表达，用SRB法检测阻抑p70S6K的表达对柚皮苷抑制细胞生长作用的影响。结果: 柚皮苷呈浓度依赖性抑制非小细胞肺癌细胞的生长，并且可以下调p70S6K的表达。过表达p70S6K可以削弱柚皮苷抑制细胞生长的作用，敲降p70S6K可以增强柚皮苷抑制细胞生长的作用。结论: 柚皮苷通过下调p70S6K抑制非小细胞肺癌细胞的生长。

Naringin inhibits the growth of non-small cell lung carcinoma cells through down-regulating p70S6K

AIM: To study the effect of naringin on the growth of non-small cell lung carcinoma cells (NSCLC) and its mechanism. METHODS: SRB method was employed to investigate the inhibitory effect of naringin on the growth of non-small cell lung carcinoma cell lines. Western blot was used to detect the p70S6K expression. The plasmid encoding full length p70S6K was transfected to over-express p70S6K. The small-interfering RNA was used to knockdown p70S6K expression. RESULTS:Naringin inhibited the growth of NSCLC cells, and down-regulated the expression of p70S6K. The over-expression of p70S6K partially abrogated the inhibitory effect of naringin, while knockdown of p70S6K enhanced the effect of naringin. CONCLUSION: Naringin can down-regulate the p70S6K to inhibit the growth of NSCLC cells.