人眼眶横纹肌肉瘤动物模型的建立

目的:探讨建立人眼眶横纹肌肉瘤动物模型的方法. 方法:培养人胚胎型横纹肌肉瘤细胞株RD细胞,取对数生长期细胞分别接种于4周龄SCID鼠和60Co照射 (1 Gy) 后1 d 的BALB/c裸小鼠;选用成瘤BALB/c裸小鼠的瘤块移植入另一批4周龄BALB/c裸小鼠胁部皮下(组织块接种法).观察各组动物肿瘤生长情况,绘制肿瘤生长曲线;肿瘤组织行常规病理H-E染色并镜检. 结果:60Co照射后1 d裸小鼠成瘤时间约6～8周,SCID鼠组约3～4周,组织块接种法移植2周后裸小鼠瘤体较明显,3组动物的成瘤率均为100%;成功绘制出肿瘤生长曲线,肿瘤均呈进行性生长;病理检查示各组移植性横纹肌肉瘤与人体肿瘤标本所见类似.结论:成功建立了裸小鼠和SCID鼠人眼眶横纹肌肉瘤模型,组织块接种法可以缩短BALB/c裸小鼠成瘤时间,为进一步研究人眼眶横纹肌肉瘤奠定了基础.

Establishment of human orbital rhabdomyosarcoma model in mice

Objective:To establish a mouse model of human orbital rhabdomyosarcoma(RD).Methods: RD cells were cultured and implanted subcutaneously into the costal regions of nude mice and severe combined immunodeficiency(SCID) mice(4 weeks old).The nude mice were radiated by ~(60)Co 1 day before the injection.The rhbdomyosarcoma tissue masses were harvested from nude mice and were subcutaneously implanted into the costal regions of another group of BALB/c mice(tumor tissue mass implantation).The tumor growth was observed in all groups and the growth curves were ploted.H-E staining was used to examine pathology of the tumor tissues.Results: Solid tumors were palpable 6-8 weeks after implantation in ~(60)Co radiated nude mice and about 3-4 weeks after implantation in SCID mice.The solid tumors were obviously seen in the nude mice 2 weeks after tumor tissue mass transplantation.The tumor forming rates were 100% in all groups.The tumor growing curves were successfully ploted and the tumors had a progressive growth.Pathological findings of the tumor tissue were similar to those of human orbital rhabdomyosarcoma. Conclusion: The human orbital rhabdomyosarcoma models have been successfully established in nude mice and SCID mice.Tumor tissue mass implantation can shorten the tumor forming time in BALB/c nude mice.This research lays a foundation for further study or human orbital rhabdomyosarcoma.