云南抗白叶枯病稻种的RGA初析

根据水稻抗白叶枯病Xa21基因的富含亮氨酸重复区域（LRR）和番茄抗细菌性斑点病(Pseudomonas syringae pv. tomato)的Pto基因编码蛋白质激酶的DNA序列，设计2对引物用于扩增抗水稻白叶枯病品种中的抗病基因同源序列。经聚丙烯酰胺凝胶电泳和聚类分析，结果表明供试抗病品种间具有丰富的RGA多态性，用同一引物测定的属于同一簇

Preliminary Analysis of Resistance Gene Analogs for Rice Cultivars to Bacterial Blight in Yunnan

Polymerase chain reaction(PCR) primers corresponding to the conserved motifs of prototype resistance genes were used to characterize rice cultivars with resistance to bacterial blight in Yunnan Province. The RGA analysis was conducted by PCR amplification using two primers, i.e. XLRR for/XLRR rev for LRR of Xa21 resistance gene to Xanthomonas oryzae pv. oryzae and Pto-kin1/Pto kin2 for protein kinase of Pto resistance gene to Pseudomonas syringae pv. tomato, respectively. The results showed that abundant RGA polymorphism was observed among the resistance cultivars tested. The cultivars which were belonged to the same cluster showed similarly resistance or spectrum of resistance to Xanthomonas oryzae pv. oryzae isolates tested. Among the cultivars clusters which were formed by RGA profile of XLRR for/XLRR rev primer, forty-six cultivars tested could be divided into 9 groups at genetic distance 0.25, group 3, 4, 7 were prodemiant, and the group 3 was divided into 5 subgroups including 23 cultivars at genetic distance 0.2. The RGA markers were useful in grouping genetically related cultivars for rice breeding to bacterial blight control and to select parental germplasm with differential genetic background.