尿中双酚S测定的涡旋辅助液液微萃取-高效液相色谱法

目的建立涡旋辅助液液微萃取-高效液相色谱法测定尿中双酚S的方法。方法以乙腈为分散剂,正辛醇为萃取剂,萃取尿中双酚S。采用单因素轮换实验确定最佳萃取条件,并评估方法学性能指标。结果尿中双酚S在0.0~160 μg/L范围线性相关系数大于0.999,检出限为0.76 μg/L,样品加标回收率为88.06%~103.81%,日内精密度范围为1.78%~2.85%,日间精密度范围为2.65%~4.25%。结论该法准确可靠,灵敏度高,适用于职业接触人群及非职业人群尿中双酚S的测定。

Determination of bisphenol S in urine by vortex-assisted liquid-liquid microextraction with high performance liquid chromatography

Objective To establish a method for the determination of bisphenol S in urine using dispersive liquid-liquid microextraction(DLLME)coupled with high performance liquid chromatography(HPLC).Methods The acetonitrile,octanol were used as extraction solvent,dispersive solvent respectively,for the preconcentration of bisphenol S.The optimal extraction conditions were optimized by single factor rotations,and methodological performance index were tested.Results The linear correlation coefficient of bisphenol S in the range of 0.0-160μg/L is greater than 0.999.The detection limit of this method was 0.76μg/L,and the recovery rates were 88.06%-103.81%.The intra-and inter-day precisions were 1.78%-2.85%and 2.65%-4.25%,respectively.Conclusion The method is reliable and sensitive.It is suitable for the determination of bisphenol S in urine samples for occupational exposure populations and non-professional.