| 胃癌细胞C-myc蛋白对他莫昔芬的应答 |
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| 引用本文： | 余怡,曾智,邓昊. 胃癌细胞C-myc蛋白对他莫昔芬的应答[J]. 江汉大学学报(自然科学版), 2008, 36(3) |
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| 作者姓名： | 余怡 曾智 邓昊 |
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| 作者单位： | [1]江汉大学医学院,武汉430056 [2]天津医科大学病理学系,天津300070 [3]江汉大学肿瘤研究所,武汉430056 |
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| 摘 要： | 目的:观察C-myc蛋白在胃癌细胞系BGC823中的表达情况,探讨胃癌细胞BGC823经他莫昔芬(TAM)处理后,C-myc蛋白表达的变化.方法:使用10 5MTAM处理胃癌BGC823细胞系24 h后,通过免疫荧光细胞化学的方法及细胞计数,检测C-myc蛋白的表达变化,并通过WST1法检测细胞活力的变化.结果:C-myc在胃癌BGC823中高表达,经10 5 M TAM处理24 h后BGC823细胞活力明显下降(P<0.05,)同时C-myc的表达减弱(P<0.05).结论:C-myc在胃癌细胞BGC823中发挥生物学作用且与TAM介导的BGC823细胞活力下降相关.
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| 关 键 词： | C-myc 胃癌 BGC823 他莫昔芬 |
Response of Protein C-myc to Tamoxifen in Grastric Cancer Cell |
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| Abstract: | To observe the expression of C-myc in gastric cancer BGC823 cell and research the alteration of C-myc expression in gastric cancer BGC823 cell after the treatment of Tamoxifen.Methods: By fluorescence immunocytochemistry staining and cell counting of BGC823 cell treated with 10 5M Tamoxifen for 24 h,detected the expression alteration of C-myc,at the same time,cell energy was detected by WST-1 method.Results:The over expression of C-myc was detected in gastric cancer BGC823 cell.The decrease of expression of C-myc(P < 0.05) and cell energy(P < 0.05) were observed in gastric cancer BGC823 cell treated with 10 5M Tamoxifen for 24 h.Conclusion:C-myc plays an important biological role in the gastric cancer BGC823 cell and is related to cell energy change mediated by Tamoxifen. |
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| Keywords: | C-myc gastric cancer BGC823 cell Tamoxifen |
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