| 拟南芥JR1基因片段的克隆及分析 |
| |
| 引用本文： | 于涌鲲,万善霞,赵福宽,孙清鹏.拟南芥JR1基因片段的克隆及分析[J].生物技术通报,2007(4):106-109. |
| |
| 作者姓名： | 于涌鲲 万善霞 赵福宽 孙清鹏 |
| |
| 作者单位： | 1. 北京农学院组培实验中心,北京,102206
2. 北京农学院生物技术系,北京,102206 |
| |
| 基金项目： | 北京市自然科学基金;北京市科技新星计划项目 |
| |
| 摘 要： | 根据拟南芥CBF基因序列的保守区设计合成一对特异引物,以菠菜基因组DNA为模板,采用PCR扩增的方法扩出一条DNA特异片段并克隆到pUC18载体中。用酶切和测序分析法对克隆片段进行鉴定并进一步进行核苷酸序列分析。序列测定该片段长为430bp。OMIGA2.0软件分析结果表明,该片段与已报道的拟南芥JR1序列的一致性为99.1%。
|
| 关 键 词： | 拟南芥 克隆 |
| 修稿时间： | 2007-03-01 |
99.1%. Cloning and Sequencing of JR1 Fragment from Arabidopsis thaliana |
| |
| Yu Yongkun,Wan Shanxia,Zhao Fukuan,Sun Qingpeng.99.1%. Cloning and Sequencing of JR1 Fragment from Arabidopsis thaliana[J].Biotechnology Bulletin,2007(4):106-109. |
| |
| Authors: | Yu Yongkun Wan Shanxia Zhao Fukuan Sun Qingpeng |
| |
| Affiliation: | 1 Center of Tissue Culture ,Beijing Agricultural College ,Beijing 102206;2 Department of Biotechnology,Beijing Agricultural College,Beijing 102206 |
| |
| Abstract: | PCR primers were designed based on the Arabidopsis JR genes.Total RNA was extracted from seedlings of Arabidopsis thanalia.One fragment was obtained by polymerase chain reaction and cloned into pUC18 vector.The clone identified was sequenced and digested with restriction enzymes.The results showed that the cloned fragment was about 430 bp in length.Sequence analysis by OMIGA2.0 showed that the sequences of the cloned fragment was 100% homologous to JR1 cDNA sequence. |
| |
| Keywords: | JR1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
