对萼猕猴桃叶中黄酮类成分的含量分析

目的：分析对萼猕猴桃叶中总黄酮以及黄酮类成分山柰酚-3-O-β-D-吡喃半乳糖苷（ry-1）、对萼猕猴桃苷E（ry-2）、对萼猕猴桃苷F（ry-3）的含量,为对萼猕猴桃叶品质评价和质量控制提供依据。方法：采用芦丁比色法对对萼猕猴桃叶中总黄酮进行含量分析;建立HPLC法对对萼猕猴桃叶中黄酮类成分ry-1、ry-2、ry-3进行含量分析。色谱柱：Shim-packCLC-ODS色谱柱（4.6mm×25mm,5μm）;保护柱：DIKMAEasy-Guard C18（10mm×4.6mm）,流动相：乙腈-0.5%醋酸水溶液梯度洗脱;柱温：26℃;检测波长：360nm;流速：1.00mL/min。结果：总黄酮的回归方程为A=8.26×10^-2＋1.90×10^-3,r=0.9995,线性范围：0.01～0.09mg/mL;ry-1回归方程为A=6.84×10^-5C＋6.53×10^-2,r=0.9999,线性范围：0.792～23.760μg/mL;ry-2回归方程为A=8.58×10^-5C＋0.124,r=0.9999,线性范围：2.570～77.100μg/mL;ry-3回归方程为A=7.89×10^-5C＋9.54×10-2,r=0.9999,线性范围：2.168～65.040μg/mL。结论：该方法前处理简便,分析准确、快速,可以作为对萼猕猴桃叶中总黄酮以及ry-1、ry-2、ry-3的定量分析方法,也为对萼猕猴桃叶的质量控制和品质评价提供了重要的依据。

Quantitative Analysis of Flavonoids in Leaves of Actinidia valvata

Objective：To determine content of total flavonoid and in leaves of Actinidia valvata,which may provide experimental evidence for its qualitative control and evaluation.Methods：Rutoside-UV method was used for the determination of total flavonoid and HPLC method was developed for the determination of kaempferol-3-O-β-D-galactopyranoside（ry-1）,actinoside E（ry-2） and actinoside F（ry-3） in leaves of Actinidia valvata.The HPLC method was developed with the conditions as following：Shim-pack CLC-ODS column（4.6mm×25mm,5μm）,DIKMA Easyguard C18 （10mm×4.6mm）,gradient elution was employed with the mobile phase acetonitrile-0.5% acetic acid solution at flow rate of 1.00mL/min;Column temperature：26 ℃;Detection wavelength：360 nm.Results：The standard curves of total flavonoid,ry-1,ry-2 and ry-3 were A=8.26×10^-2C＋ 1.90×10^-3 （r=0.9995）,A=6.84×10^-5C ＋6.53×10^-2 （r=0.9999）,A=8.58×10^-5C＋0.124（r=0.9999） and A=7.89×10^-5C ＋9.54×10^-2 （r=0.9999）,respectively.The standard curves of total ry-1,ry-2 and ry-3 were linear in the range of 0.01～0.09 mg/mL,0.792～23.760 μg/mL,2.570～77.100μg/mL and 2.168～65.040μg/mL,respectively.Conclusion：The method is feasible for quantitative analysis of total flavonoid,ry-1,ry-2 and ry-3 in leaves of Actinidia valvata,and also provided meaningful evidence for its qualitative control and evaluation.