冠心病患者内皮祖细胞tPA和PAI表达的变化

目的探讨冠心病患者外周血中内皮祖细胞(EPC)的变化及其组织型纤溶酶原激活物(tPA)和纤溶酶原激活剂抑制剂(PAI)的表达。方法选择冠心病患者57例和对照组30例,提取内皮祖细胞进行数量和细胞集落的比较。利用ELISA法和底物发光法检测EPC分泌tPA和PAI的浓度和活性;用RT-PCR法检测EPC的tPA和PAI mRNA表达。结果冠心病患者EPC数量较对照组明显减少(23.1±1.8比56.7±2.4,P<0.05),形成细胞集落数(14.7±2.5比24.2±1.7,P<0.05)、细胞增殖能力也明显降低,冠心病患者EPC的tPA表达较对照组下降,PAI表达增强。结论冠心病患者外周血EPC数量减少和功能障碍可能在疾病的发生发展中起作用。

Reseach of Endothelial Projenitor Cells from Prepheral Blood in Patients with Coronary Heart Diseases

Aim To study the change of EPC and expression of its tPA and PAI in patients with coronary heart disease. Methods EPC isolated from peripheral blood of patients with coronary heart diseases(n57) and control (n30) were cultured and tPA and PAI in the supernatant were determined by ELISA and Substrate chemiluminescence.The expression of tPA mRNA and PAI mRNA were detected by RT-PCR. Results The number of EPC was significantly reduced in patients with CAD compared with control subjects. The cell colony forming was also decreased in patients with CAD. The concentration and activity of EPC patients with CAD were lower than EPC of control group. The tPA mRNA expression, the concentration and activity of EPC had degression tendency in patients with coronary heart diseases, and PAI displayed opposite tendency. Conclusion The number of EPC were decreased and their function were impaired in patients with CAD, which may play an important role in the initiation and progression of CAD.