5’-单磷酸腺苷在乙醇-水中介稳区性质研究

针对5’-单磷酸腺苷(5’-AMP)在溶析结晶中易出现爆发结晶的问题,分别采用静态法和激光法测定了293—323 K下5’-AMP在纯水以及溶剂体积比为0.3—2.0的乙醇-水体系中的溶解度和超溶解度,以指数经验关联式建立了超溶解度关联式,并在试验条件下考察了温度、乙醇流速、pH值和搅拌速率对介稳区宽度的影响。运用中心复合设计预测了5’-AMP在本溶析结晶体系中介稳区间,并用扫描电镜观察了5’-AMP在结晶条件优化前后晶体形态的变化情况,为工业生产中提高结晶产品质量提供了理论指导。

Research on metastable zone of adenosine 5'-monophosphate in ethanol and water

Qualitative data on the solubility and supersolubility for binary system 5'-AMP H2O solution and ternary system 5'-AMP H2O ethanol solution was determined at 293-323 K in the volume fraction ethanol range of 0.3-2.0,which were essential for solvent-out crystallization studies.The supersolubility of 5'AMP fulfills well the empirical formula in both pure water and H2O ethanol solution.Temperature,ethanol addition rate,pH levels and rotate speed of stirrer were being recognized as nonvasive control parameter for adenosine 5'-monophosphate crystallization.The metastable zone was obtained under the conditions of temperatures,ethanol addition rate,pH levels and rotate speed of stirrer and estimated by using Central Composite Designs in the solvent-out crystallization process,and the variation of crystal habit of 5'-AMP was studied by scanning electron microscope after optimization of the crystallization process.All the results were important for the control of industrial crystallization of 5'-AMP.