| RECK基因在前列腺细胞株中的表达及其与MMP-2的关系 |
| |
| 引用本文： | 徐振宇,孙颖浩,高建平,张征宇,葛京平,许传亮,王林辉. RECK基因在前列腺细胞株中的表达及其与MMP-2的关系[J]. 江苏医药, 2007, 33(4): 372-374 |
| |
| 作者姓名： | 徐振宇 孙颖浩 高建平 张征宇 葛京平 许传亮 王林辉 |
| |
| 作者单位： | 210002,南京军区南京总医院泌尿外科;上海第二军医大学长海医院泌尿外科 |
| |
| 摘 要： | 目的 检测RECK基因在不同前列腺细胞株BPH-1、DU-145、LNCap以及PC-3中的表达,探讨其表达差异及其与基质金属蛋白酶2(MMP-2)的关系.方法 应用RT-PCR技术及Real-time PCR技术检测4种不同前列腺细胞株中RECK基因及MMP-2 mRNA的表达;应用Western blot检测不同前列腺细胞株中RECK基因的表达.结果 前列腺癌细胞株DU-145、LNCap以及PC-3中RECK mRNA的表达较良性前列腺增生细胞株BPH-1中明显降低(P＜0.01),MMP-2 mRNA表达则明显升高.DU-145、LNCap以及PC-3细胞株中RECK基因的表达较正常组织明显降低.结论 RECK基因在前列腺癌中的表达量明显下降,而MMP-2的表达量明显升高,RECK基因可能通过抑制MMP-2的表达起到抑癌基因的作用.
|
| 关 键 词： | 前列腺癌 RECK基因 基质金属蛋白酶2 |
| 文章编号： | 0253-3685(2007)04-0372-03 |
| 修稿时间： | 2006-06-06 |
The expression of MMP inhibitor RECK gene in the prostate cell strains |
| |
| XU Zhenyu, SUN Yinghao, GAO Jianping ,et al.. The expression of MMP inhibitor RECK gene in the prostate cell strains[J]. Jiangsu Medical Journal, 2007, 33(4): 372-374 |
| |
| Authors: | XU Zhenyu SUN Yinghao GAO Jianping et al. |
| |
| Affiliation: | Department of Urology, Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, CHINA |
| |
| Abstract: | Objective To investigate the role of PECK gene in the progression and invasion in the prostate carcinoma.Methods The mRNA level was measured by RT-PCR and real-time PCR,the protein level was evaluated by Western blot.Results The mRNA level of the PECK gene on the prostate carcinoma cell strains(DU-45,LNCap and PC-3) was lower than that on the benign prostate hyperplasia cell strain BPH-1,while the matrix metalloproteinase-2 (MMP-2) level was higher.Also the protein level of RECK on the DU-45,LNCap and PC-3 was lower than that on the BPH-1.Conclusion PECK gene was supposed to be a kind of tumor suppression gene,which may act by inhibiting the activity of MMPs. |
| |
| Keywords: | Prostate carcinoma PECK gene MMP-2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
