补肾醒脑解毒方对培养神经元单纯疱疹病毒感染后NGF和BDNF表达的影响

观察补肾醒脑解毒中药复方对HSV-1感染后神经元内NGF、BDNF表达的调节作用.用HSV-1感染原代培养大鼠海马神经元造成急性感染与潜伏感染,然后运用Northern杂交方法检测中药煎液对感染神经元NGF和mRNA表达水平的影响.结果显示(1)正常培养海马神经元内有微量NGF和BDNF表达,加入中药煎液后NGF和BDNF表达明显提高;(2)感染0.1MOI HSV-1后6-12h出现BDNF与NGF表达增加,24h后下降,48h后显著低于正常水平,4d后表达消失.加中药煎液后NGF和BDNF表达时间延长;(3)补肾醒脑解毒方能使潜伏感染神经元NGF和BDNF表达持续增加,并维持潜伏感染神经元存活达10周以上.结果表明补肾醒脑解毒方对HSV-1感染神经元NGF和BDNF表达具有上增性调节作用.

The Effects of Bushen Xingnao Jiedu Fang on Regulating Expressions of NGF and BDNF in Cultured Hippocampal Neurons After Infection of Herpes Simplex Virus Type 1

Observing the effects of Bushen Xingnao Jiedu Fang(BXJF) on regulating the expressions of nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) in cultured hippocampal neurons after infection of herpes simplex virus type 1(HSV 1) in vitro.Detecting the expression level of NGF mRNAs in normal cultured neurons,acute infection neurons,latent infection neurons,and BXJF treated infected neurons with Northern blot.Results indicated:(1)Expressions of NGF and BDNF mRNAs were observed on hippocampal neurons and the expressions were increased after addition of the decoction of BXJF.(2)A transient increasing expression of NGF and BDNF mRNAs was also found from 6h to 12h after HSV 1 infection.But expression level of NGF mRNA and BDNF mRNA could not be detected at day 4 after HSV 1 infection.The time courses of NGF and BDNF expressing were extended for two days.(3)The increasing expressions of NGF mRNA and BDNF mRNA in neurons with HSV 1 latent infection were remained by addition of the decoction of BXJF,and these neurons survivd for at least ten weeks after deprivation of acyclovir.The results indicated the decoction of BSXNJDF was found to have the effects on increasing expressions of NGF mRNA and BDNF mRNA in normal cultured neurons and HSV infected neurons.