| 高固液比体系中酶催化合成Z-Phe-Leu-OMe的研究 |
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| 引用本文： | 黄振龙,宗璟,陈英文,沈树宝.高固液比体系中酶催化合成Z-Phe-Leu-OMe的研究[J].食品与生物技术学报,2006,25(2):20-23. |
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| 作者姓名： | 黄振龙 宗璟 陈英文 沈树宝 |
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| 作者单位： | 南京工业大学,制药与生命科学学院,南京,210009 |
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| 基金项目： | 中国科学院资助项目;江苏省自然科学基金 |
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| 摘 要： | 以模型二肽Z—Phe—Leu—OMe的合成为对象讨论了高固液比条件下酶催化体系的性质。研究了固液比高低对二肽产率的影响，测定了高固液比条件下随着反应进行体系pH的变化，尝试提出该类体系pH变化的机理，并采用KHCO3和CaCO3调节该体系的pH来提高催化效率。
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| 关 键 词： | 高固液比 模型二肽 酶催化 pH值 |
| 文章编号： | 1673-1689(2006)02-0020-04 |
| 修稿时间： | 2004年12月13 |
Enzymatic Synthesis of Z-Phe-Leu-OMe in A High Solid-Liquid Ratio System |
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| HUANG Zhen-long,ZONG Jing,CHEN Ying-wen,SHEN Shu-bao.Enzymatic Synthesis of Z-Phe-Leu-OMe in A High Solid-Liquid Ratio System[J].Journal of Food Science and Biotechnology,2006,25(2):20-23. |
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| Authors: | HUANG Zhen-long ZONG Jing CHEN Ying-wen SHEN Shu-bao |
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| Abstract: | Enzymatic synthesis of model dipeptide Z-Phe-Leu-OMe in a high solid-liquid ratio system was investigated.Effects of solid-liquid ratio on the dipeptide yield were analyzed.In addition,the pH change of the system during the reaction was monitored,and the mechanism of this change was presented.The catalytic efficiency of Z-Phe-Leu-OMe enzymatic synthesis was further promoted by adjusting pH of the system through addition of KHCO_(3) and CaCO_(3). |
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| Keywords: | high solid-liquid ratio model dipeptide enzymatic catalysis pH value |
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