| 离心淘洗技术在分离大鼠肝非实质细胞亚群中的应用 |
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| 引用本文： | 钱学敏,邱德凯,李恩灵,范竹萍. 离心淘洗技术在分离大鼠肝非实质细胞亚群中的应用[J]. 肝脏, 2004, 9(1): 11-14 |
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| 作者姓名： | 钱学敏 邱德凯 李恩灵 范竹萍 |
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| 作者单位： | 200001,上海第二医科大学附属仁济医院,上海市消化疾病研究所;200001,上海第二医科大学附属仁济医院,上海市消化疾病研究所;200001,上海第二医科大学附属仁济医院,上海市消化疾病研究所;200001,上海第二医科大学附属仁济医院,上海市消化疾病研究所 |
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| 基金项目： | 上海市自然科学基金，上海市重点学科建设项目资助(0 1ZB1 4 0 2 7) |
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| 摘 要： | 目的：采用离心淘洗技术分离纯化大鼠肝非实质细胞亚群。方法：通过原粒肝脏酶灌注，准备肝非实质细胞悬液，离心淘洗，收集不同流速(20和40ml／min)的细胞群。结果：经培养鉴定，流速21ml／min获得的为肝窦内皮细胞，40ml／min的为库普弗细胞，细胞纯度均大于95％。结论:在本实验室，离心淘洗技术已能成功应用于肝脏细胞分离过程。
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| 关 键 词： | 离心淘洗 肝窦内皮细胞 库普弗细胞 |
| 修稿时间： | 2004-02-09 |
Application of centrifugal elutriation technique for isolation of rat liver nonparenchymal cells subgroups |
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| QIAN Xue min,QIU Dekai,LI Enlin,et al.. Application of centrifugal elutriation technique for isolation of rat liver nonparenchymal cells subgroups[J]. Chinese Hepatology, 2004, 9(1): 11-14 |
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| Authors: | QIAN Xue min QIU Dekai LI Enlin et al. |
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| Affiliation: | QIAN Xue min,QIU Dekai,LI Enlin,et al. Shanghai Second Medical University Renji Hospital,Shanghai Institute of Digestive Disease Shanghai 200001 China |
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| Abstract: | Objective In order to isolate and purify nonparenchymal cells subgroups from rat liver, centrifugal elutriation technique was applied. Methods By the procedures of liver perfusion in situ, preparation of nonparenchymal cells suspension and centrifugal elutriation, cell fractions from different flow rate were collected.Results According to primary culture and identification, sinusoidal endothelial cells were contained from a flow of 21 ml/min, Kupffer cells were got 40ml/min, the cells purity were both more than 95%. Conclusion Centrifugal elutriation technique could be applied successfully during the separation of liver cells in our laboratory. |
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| Keywords: | Centrifugal elutriation Sinusoidal endothelial cells(SECs) Kupffer cells |
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