深圳市淡水鱼华支睾吸虫感染调查及实验研究

目的了解深圳市淡水鱼华支睾吸虫(肝吸虫)囊蚴感染情况,阻断和控制肝吸虫病在深圳市的传播和流行。方法用鱼体消化法筛查肝吸虫囊蚴;以肝吸虫过氧化物酶(SOD)基因保守序列作为摸板,RT- PCR鉴别肝吸虫囊蚴。结果对深圳市淡水鱼肝吸虫感染情况进行调查,共抽检16种鱼257份样品,其中检出肝吸虫囊蚴45份,检出率为17．50％。检出率最高的鱼种依次为鲩鱼40．74％、鲮鱼26％、鲤鱼22．5％、大头鱼 22．85％、生鱼16．6％、非洲鲫鱼14．5％;RT-PCR检测结果与镜检结果完全一致。结论检测结果显示深圳市淡水鱼中肝吸虫的感染情况严重,尤其是鲩鱼的感染率较高,为了控制和阻断肝吸虫的传播必须改变吃淡水鱼生的生活习惯。

Investigation of Clonorchis sinensis Infection in Freshwater Fish in Shenzhen City

Objective To investigate infection situation of Clonorchis sinensis infection in freshwater fish in Shenzhen area, and to control the transmission and spread of Clonorchis sinensis in Shenzhen area. Methods Freshwater fishes from Nanshan, Futian, Yantian, Luohu, Baoan and Longgang were examined for metacercaria of Clonorchis sinensis using digestion method and PCR. Results The encysted metacercaria of Clonorchis sinensis were found in the muscles of freshwater fishes collected from the above six districts.The situation of metacercaria of Clonorchis sinensis infection in freshwater fishes was the most serious in Longgang and less serious in Luohu. The infection rate of campo was the highest in all kinds of the fish. Conclusion The infection situation of Clonorchis sinensis in feshwater fish from Shenzhen area was quite severe and the control of Clonorchis sinensis infection must be enforced.