New generation IQ‐200 automated urine microscopy analyzer compared with KOVA cell chamber |
| |
Authors: | Emel Altekin Ozgur Kadiçesme Pinar Akan Tuncay Kume Ozgul Vupa Gul Ergor Hakan Abacioglu |
| |
Affiliation: | 1. Department of Biochemistry and Clinical Biochemistry, Dokuz Eylul University, Izmir, Turkey;2. Department of Statistics, Faculty of Science and Letters, Dokuz Eylul University, Izmir, Turkey;3. Department of Public Health, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey;4. Department of Microbiology and Clinical Microbiology, Dokuz Eylul University, Izmir, Turkey |
| |
Abstract: | Objective: The examination of the urine remains to be one of the most commonly performed tests in laboratory practice. Currently, laboratories also need to accredit their urine diagnostics by comparing their measurement methods to acceptable references. In this study we compared particle counts obtained by new generation automated technique, image capture analysis (IQ‐200) with those of a standardized chamber counts. Design and Methods: The same 258 urine samples from different departments of a hospital assayed by IQ‐200 were analyzed in parallel with the KOVA cell chamber system. Clinically significant discrepancy results (positive vs. negative) for red blood cell (RBC) and white blood cell (WBC) were also compared with those obtained by dipstick testing. Results: There was a good agreement between the automated system and sediment microscopy for RBCs, WBCs, and squamous epithelial cells (SCs) (r=0.90; r=0.80; r=0.72, respectively: P<0.001). The IQ‐200 was more sensitive for determining RBCs, WBCs, and SCs than other formed elements. Conclusions: IQ‐200 can perform accurate quantification of microscopic element in urine. However, automated techniques are not completely free of error. Therefore, by adopting an appropriate algorithm and combining the results with stript analysis and other laboratory tests allows further reduction of clinically important errors. J. Clin. Lab. Anal. 24:67–71, 2010. © 2010 Wiley‐Liss, Inc. |
| |
Keywords: | automated analyzer chamber counting urine analysis |
|
|