维生素D与基质金属蛋白酶-9在慢性阻塞性肺疾病中的表达及相关性分析

目的检测COPD患者血清中25羟维生素D-25-hydroxyvitaminD，25-（OH）D]与基质金属蛋白酶-9（matrixmetalloproteinase-9，MMP-9）的表达水平并探讨两者之间的关系。方法收集2012年1月至2012年3月在长沙市第三医院呼吸内科住院的38例COPD急性加重期患者（COPD急性加重组）和同期同院门诊随诊的40例COPD稳定期患者（COPD稳定组），同时选择30例在同院体检中心健康体检者（对照组）。采用ELISA法测定并比较3组受试者血清25-（OH）D和MMP-9的表达水平，分别探讨c（）PD稳定组和急性加重组中血清25-（OH）D和MMP-9水平之间的关系。结果①3组的血清25～（OH）D水平比较，cOPD急性加重组和COPD稳定组分别低于对照组（F=86．63，P〈0．01），COPD急性加重组低于COPD稳定组（F=86．63，P〈0．05）；3组的血清MMP-9水平比较，COPD急性加重组和COPD稳定组分别高于对照组（F=436．02，PG0．01），COPD急性加重组高于以）PD稳定组（F=436．02，PG0．01）。②COPD稳定组25～（OH）D与MMP-9水平呈负相关（r=-0．638，PG0．01）。COPD急性加重组25（OH）D与MMP-9水平呈负相关（r=-0．579，PG0．01）。结论维生素D在C（）PD中呈低表达而MMP-9呈高表达。在COPD患者中，维生素D水平可能会影响MMP-9的表达。

Expression and correlation of vitamin D and MMP-9 in chronic obstructive pulmonary disease

Objective To detect the levels of 25-hydroxyvitamin D 25-（OH） D] and matrix metalloproteinase-9 （MMP-9） in COPD and to analyze the correlations of these indices. Methods Thirty- eight patients with acute exacerbations of COPD （AECOPD group）,40 outpatients with stable COPD （stable COPD group） and 30 healthy subjects（control group） in the Third Hospital of Changsha were enrolled in our study from Jan. 2012 to Mar. 2012. Serum levels of 25-（OH）D and MMP-9 were measured in all subjects by the ELISA and compared among three groups. Correlations between 25-（OH）D and MMP 9 were analyzed in stable COPD group and AECOPD group. Results @The levels of 25-（OH）D in AECOPD group and stable COPD group were significantly lower than those in control group （ F = 86.63, P %0.01）. The level of 25-（OH）D in AECOPD group was significantly lower than that in stable COPD group （ F =86.63, P %0.05）. The levels of MMP-9 in AECOPD group and stable COPD group were significantly higher than those in control group （F = 436.02, P %0.01）. The level of MMP-9 in AEC（）PD group was significantly higher than that in stable COPD group （ F = 436.02, P %0.01 ）. （2） Both in stable COPD group and AECOPD group,the 25-（OH）I） levels were negatively correlated with the MMP-9 levels respectively （ r = -0. 638, P %0.01. r = -0. 579, P %0.01, respectively）. Conclusions Vitamin D in COPD showed lower expression, while higher expression of MMP-9. Vitamin D may affect the expression of MMP-9 in COPD.