生殖激素水平在男性不育患者Y染色体微缺失中的研究

目的筛查严重少精子症和无精子症患者Y染色体AZF区域微缺失的发生情况，探讨Y染色体微缺失患者生殖激素的水平。方法对195例严重少精子症和80例无精子症患者进行Y染色体无精子因子（azoospermia factor，AZF）微缺失分析，同时用化学发光法测定生殖激素水平。结果275例患者中发生AZF微缺失患者21例，检出率为7．6％，其中少严重精子症15例，无精子症6例。21例AZF微缺失情况：AZFa区缺失3例；AZFb＋c＋d区缺失4例；AZFc＋d区缺失¨例；AZFd区缺失3例。Y染色体AZFb＋C＋d区缺失患者的卵泡刺激素（FSH）值（46．2±10．3）mIU／mL显著高于无Y染色体缺失患者（17．6±15．2）mIU／mL和AZFa区、AZFc＋d区、AZFd区缺失患者（15．8±5．7）mIU／mL，差异具有统计学意义（P〈0．05）。结论在无精与严重少精症患者中Y染色体的微缺失以AZFc区和AZFd区缺失最为常见，Y染色体AZFb＋c＋d区缺失是引起高卵泡刺激素的重要原因之一。

Analysis of gonadal hormone level in male infertility patients of Y chromosome microdeletion.

Objective： To screen for Y chromosome microdeletions in patients with severe oligospermia or azoospermia, and ex- plore the levels of gonadal hormone about patients of Ychromosome microdeletion. Methods： Y chromosome microdeletions among 195 patients with severe oligospermia and 80 patients with azoospermia were analyzed, and the levels of gonadal hormone were detected by chemilu - minescent met - hod. Results ： The rate of microdeletion was 7. 6% （21 out of 275 patients）, of which 15 were severe oli-gospermia, 6 were azoospermia. The deletion was on AZFa in 3 patient, AZFb ＋ c ＋ d in 4patients, AZFc ＋ d in 11 patients, AZFd in 3 patient, respectively. The level of FSH （46. 2 ＋ 10. 3mIU/mL） in the AZFb ＋ c ＋ d deletion group was significantly higher than that in the group without Y chromosome deletion （17.6 ± 15.2mIU/mL） and the other types of deletion （ 15. 8 ± 5.7 mlU/mL） （ P 〈 0. 05 ）. Conclusion ： The common microdeletion regions were AZFc and AZFd on Y chromosome in azoospermia or severe oligozoospermia. The microdeletion of AZFb ＋ c ＋ d was one of the important causes of the high level of FSH.