超分辨显微成像技术在细胞器相互作用研究中的应用（特邀）

观察细胞器间动态相互作用，深入分析作用规律，对于揭示生理病理过程现象背后的机制具有十分重要的意义。传统光学显微镜受到由光波波长和孔径造成的衍射极限的限制，无法观测细胞器纳米级精细结构及细胞器间相互作用的动态变化规律。超分辨显微成像技术的出现为细胞器相互作用研究提供了重要手段，在深入揭示细胞器相互作用规律，阐明生理病理现象深层的机制研究中发挥了重要的作用。文中介绍了受激发射损耗（Stimulated emission depletion, STED）显微成像、结构光照明显微成像（Structured illumination microscopy, SIM）、单分子定位显微成像（Single molecule localization microscopy, SMLM）技术，并总结了这三类超分辨显微成像技术在细胞器相互作用中的应用与现状，为超分辨显微成像技术在细胞器相互作用研究中的应用提供思路拓展。最后，对超分辨显微成像技术在细胞器相互作用研究中的优势与不足进行分析总结，展望了超分辨显微成像技术在活细胞内细胞器相互作用成像中的需求发展趋势，为光学与医学及生物学的交叉融合发展提供一定的参考。

Application of super-resolution microscopy in the study of organelle interactions (invited)

Observing dynamic interaction between organelles and analyzing the law of action is of great significance for revealing the mechanism behind the phenomenon of physiological and pathological processes. Due to the limitation of the optical diffraction determined by wavelength and aperture, traditional optical microscopes cannot observe the nanoscale fine structure of organelles and the dynamic changes of interactions among them. The emergence of super-resolution microscopy imaging technology provides an important mean for the study of organelle interaction. This paper introduces the fluorescence microscopy (STED), structured illumination imaging (SIM), and single-molecule localization imaging (SMLM). The application of these super-resolution microscopy in the study of dynamic interaction between organelles provides the expansion of application ideas for super-resolution microscopy. Finally, the advantages and disadvantages of super-resolution microscopy in the study of organelle interactions are analyzed. In conclusion, the demand and development trend of super-resolution microscopy technology in the imaging of intracellular organelle interaction in living cells is prospected, which provides a certain reference for the cross-integration development of optics, medicine and biology.