单精子注入卵胞浆过程对胚胎发育的影响

目的探讨卵胞浆内单精子注射（ＩＣＳＩ）操作过程对胚胎发育的影响。方法　选取５３个体外受精－胚胎移植治疗不 育症周期为对象，将同一周期的同胞卵子分为两组，分别进行ＩＣＳＩ和常规体外受精（ＩＶＦ），比较两组的胚胎发育情况。结 果ＩＣＳＩ组受精率７８％，显著高于常规ＩＶＦ组６７％的受精率，但卵子的溶散率亦高（８％νｓ３．４％）；ＩＣＳＩ组的优良胚胎数 与ＩＶＦ组相比，差异不显著（Ｐ＞０．０５），但碎片率大于５０％的胚胎数前者与，后者差异具有显著性（Ｐ＜０．０１）；移植后ＩＣＳＩ组 可供冻融的胚胎数较常规ＩＶＦ组少。结论ＩＣＳＩ操作对卵子及其后的胚胎发育潜能有影响。

Impact of intracytoplasmic sperm injection procedure on embryonic development

Objective: To evaluate the interference of intracytoplasmic sperm injection (ICSI) procedure in the embryonic development. Methods Fifty-three cycles of in vitro fertilization (IVF) were selected, and sibling oocytes were fertilized with conventional IVF or ICSI respectively. Results In ICSI group, the dissolved oocytes rate and fertilization rate were significantly higher than those of IVF group (P<0.01). The number of high quality embryos in ICSI group was similar to that of IVF group, but the number of embryos with more than 50% debris in ICSI group was remarkably higher than that of IVF group (P<0.01). The number of frozen embryos in ICSI group was smaller than that of IVF group (P<0.01). Conclusion ICSI procedure may affect embryonic development in IVF-ET.