乙肝血清学标志物定量与定性检测的对比研究

目的探讨时间分辨荧光免疫分析法（TRFIA）与酶联免疫吸附试验（ELISA）法在检测乙肝病毒（HBV）五项血清学标志物方面的关系及临床应用。方法应用TRFIA法和ELISA法分别对102例随机血清样本进行乙肝病毒五项血清学标志物定量与定性检测。结果TRFIA法检测结果出现了比ELISA法更多种类的模式,且TRFIA法检测结果每项阳性例数均多于ELISA法。结论在检测乙肝病毒（HBV）五项血清学标志物方面,时间分辨荧光免疫分析法（TRFIA）比酶联免疫吸附试验（ELISA）法更具灵敏性和特异性,结果模式更加符合患者真实情况,而且TRFIA法可定量、具有试剂稳定无放射性污染等优点,更加具有临床应用价值。

Comparison and investigation of qualitative detection and quantitative detection of the serological markers of hepstitis B

Objective To investigate the relationship and clinical application of time resolved luoro-immunoassay;time-resolved fluoroimmunoassay (TRFIA) and enzyme-linked immunoassay (ELISA) in detecting the five serological markers of hepatitis B.Methods The serological markers of hepatitis B of 102 stochastic serum samples were qualitatively and quantitatively measured respectively by TRFIA and ELISA.Result The result in TRFIA shows more patterns and positive samples than ELISA.Conclusions The result of TRFIA is more sensitive and specific than ELISA,the pattern of result more accord with the real situation of the patients.And the TRFIA can be quantitative,reagents of which can be stable without radioactivity pollution,including more value of clinical applications.