RAPD技术用于结核病交叉感染的鉴别

目的：调查RAPD技术用于结核菌株DNA指纹鉴定的效果。方法：采用多重PCR方法对一组2例母女同时发病的肺结核患者和一组2例先后发病的兄妹肺结核患者的病原菌进行了菌种鉴定。采用RAPD技术对它们的基因组DNA进行指纹多态性分析。结果：4株病原菌均为结核分支杆菌。经RAPD分型，其中母女组2菌株的DNA指纹图谱完全相同，兄妹组2菌株的DNA指纹图谱有所不同。证明母女组所染病原菌的同源性很好，很可能为同一感染源，母女之间交叉感染的可能性很大；兄妹组病原菌的同源性较差，为非交叉感染。结论：RAPD技术适用于结核分支杆菌流行病学调查，特别是交叉感染的鉴别。

Identifying study on cross infection of tuberculosis by RAPD analysis

Objective:To investigate the identifying effect of RAPD on DNA fingerprinting of M. tuberculosis. Methods: Multi PCR was adapted to identify the strains from a mother and a daughter who were infected with tuberculosis at the same time and a brother and a sister who were infected one after another.RAPD was used for analyzing fingerprinting polymorphism of genomic DNA of these strains.Results: The four pathogenic strains were all belonged to M tuberculosis.No discrimination of DNA fingerprinting was found between the strain from the mother and the daughter and a obvious discrimination was found between the strains of the brother and the sister by RAPD ANALYSIS.It proved that the homogeneity of strains isolated from the mother and the daughter was good. The contagion was the same and it might be a cross infection between the mother and the daughter. the homogeneity of strains isolated from the brother and sister was poor,it can not be a cross infection. Conclusion: The study showed that RAPD could be used for epidemiological investigation of M tuberculosis, especially for indentifying cross infection.